Quantum-si Incorporated

University of British Columbia

Boreal Genomics Corp.

Matthew Wiggin

Andrea Marziali

Joel Pel

Milenko Despotovic

Gosuke Shibahara

Valentina S Vysotskaia

Valentina Semenovna Vysotskaia

The invention includes methods and apparatus for separating mutations, especially rare and unknown mutations, using heteroduplex binding proteins. Nucleic acids may optionally be nicked at or near the mutation in order to promote heteroduplex binding protein recognition and binding. In particular, using the disclosed methods, it is possible to separate heteroduplexed nucleic acid strand pair from homoduplexed nucleic acid strand pairs having similar sequences and being at a much higher concentration. Once the heteroduplexed nucleic acids are isolated and recovered, it is straightforward to analyze the sequences of the heteroduplexed nucleic acids, e.g., using sequencing or hybrid assays.

Method for isolating target nucleic acid using heteroduplex binding proteins

The invention discloses methods and apparatus for characterizing trace nucleic acids that are biomarkers for disease. The methods and apparatus provide increased sensitivity to such trace nucleic acids, and allow analysis of nucleic acids present in a sample at only 0.01% of the wild-type sequences. The methods and apparatus are also designed for straightforward multiplexing, thus allowing pooling of clinical samples.

Biomarker anaylsis using scodaphoresis

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Vancouver, Canada

Matthew Wiggin