Codex Dna, Inc.

Telesis Bio Inc.

University of Utah Research Foundation

Synthetic Genomics, Inc.

Navigen, Inc.

The United States Of America—air Force

Matthew T Weinstock

Christopher Wilson

Eric D Hesek

Daniel Glenn Gibson

Daniel Strimling

Debra Muir Eckert

Michael S Kay

Brett D Welch

Michael Thomas Jacobsen

Tracy R Clinton

Christopher M Wilson

The present disclosure relates to the seminal discovery of a generation and use of genetically engineeredsp. Provided is the use of the genetically engineered bacteria for the construction, maintenance, manipulation, and/or propagation of DNA constructs; protein expression; protein secretion; vectors and other metabolic tools; metabolic engineering; expression of cellular extracts for cell-free biology; shuttle vectors; cloning vectors; and for synthetic biology applications. The disclosure also relates to the use of the replication machinery ofsp. as a cloning or expression vector for replication of recombinant DNA constructs. The disclosure also relates to methods of use of the above.

Genetically engineeredsp. and uses thereof

The invention provides engineeredsp. organisms that comprise a genetic modification to either or both of the lpxL and/or lpxM genes. The organisms score substantially lower in an in vitro endotoxin assay versus the unmodified or wild type organism. The organisms preserve substantially the growth rate of the corresponding unmodified organisms. The organisms can also have an exogenous nucleic acid cloned in the organism, or an exogenous nucleic acid encoding a protein, polypeptide, or peptide expressed by the organism, and optionally secreted from the organism.

Methods of cloning nucleic acids or producing proteins in a low endotoxin organism

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The present disclosure provides engineeredsp. organisms. The organisms can be engineered to have an altered Chromosome II so that the altered Chromosome II can be used insp. and other organisms for the cloning or amplification of nucleic acid molecules and for the expression and production of proteins and peptides in asp. organism. One or more genetic elements have been deleted from Chromosome II and/or relocated from Chromosome II to Chromosome I. The engineeredsp. organisms of the invention can also have signal sequences fused to proteins or peptides to be secreted from the cell. In some embodiments the engineeredsp. organisms can have sequences that enable them to retain viability after incubation at low temperatures.

Genetically engineered Vibrio sp. and uses thereof

Recombinant Vibrio natriegens organisms having a modified outer membrane

is a highly lethal filovirus that causes hemorrhagic fever in humans and non-human primates. With no approved treatments or preventatives, the development of an anti-therapy to protect against natural infections and potential weaponization is an urgent unmet global health need. The design, biophysical characterization, and validation of peptide mimics of theN-trimer ('N-trimer mimics') are described herein.

Ebolavirus pre-hairpin intermediate mimics and methods of use

Growing community of inventors

San Diego, CA, United States of America

Matthew T Weinstock