Perkinelmer Health Sciences, Inc.

Micronics, Inc.

Matthew Scott Bragd

C Frederick Battrell

Isaac Sprague

Justin L Kay

Jason Capodanno

Andrew Kolb

Alan K Lofquist

Martha Stone

Asa Bergdahl

Daniel Hoffman

Rafael Rivera

Frederick Battrell

Megan Couch

A microfluidic mixing device comprising two bellows pumps (), microfluidic cartridges comprising the same and methods for use of the same are provided. The disclosed device enables efficient mixing of samples at the microfluidic scale. More particularly, the microfluidic mixing device comprises: a first bellows pump (); a second bellows pump (); a first microchannel fluidly interconnecting the first bellows pump () with a sample inlet and a reagent reservoir, wherein the first microchannel comprises a valve (V) interposed between the pump and the inlet, and a valve (V) interposed between the pump and the reservoir; a second microchannel fluidly interconnecting the first bellows pump () with the second bellows pump (), wherein the second micro channel comprises a valve (V) interposed between the first and second pump; a third microchannel fluidly interconnecting the first bellows pump () with the second bellows pump, wherein the third micro channel comprises a valve (V) interposed between the first and second pump; a first and second pneumatic member pneumatically connected to the first and second bellows pumps; wherein, the volume of the second bellows pump () is greater than the volume of the first bellows pump ().

Microfluidic mixing device and method

Microfluidic circuit elements, such as a microvalve, micropump or microvent, formed of a microcavity divided by a diaphragm web into a first subcavity bounded by a first internal wall and a second subcavity bounded by a second internal wall, where the diaphragm web is characterized as a thin film having a first state contacting the first internal wall and a second state contacting the second internal wall and exhibiting essentially no elasticity in moving between the first state and the second state, the thin film web having been stretched beyond its yield point before or during use are provided. The disclosed elements enable faster and more efficient cycling of the diaphragm in the microcavity and increases the diaphragm surface area. In a preferred embodiment, the microfluidic circuit element is pneumatically driven and controls the motion of fluids in a microassay device.

Low elasticity films for microfluidic use

Fluidic cartridges, and manufacture thereof, having a plurality of circuit element subtypes containing pneumatically operated diaphragm members, where the diaphragm materials are selected for yield point, chemical resistance, breathability and other properties individually according to the fluidic element subtype are provided. A process of in-situ edge-bonded decoupage for forming diaphragm members inside a cartridge, and fluidic circuits having diaphragm members as active and passive circuit elements, including pumps, valves, vents, waste receptacles, reagent reservoirs, and cuvettes with optical windows, where the material composition of each individual diaphragm member may be selected from an assortment of materials during manufacture are also provided.

Fluidic circuits and related manufacturing methods

A microfluidic cartridge and methods for performing a diagnostic, molecular or biochemical assay thereon, where all dried and/or liquid reagents necessary for the assay are contained in the cartridge and the assay requires only the addition of sample. Pneumohydraulic features, chamber and diaphragm technologies are introduced for overcoming the problems of bubble interference and reagent washout during operation of a microfluidic cartridge. The cartridges are inserted into a host instrument for performance of an assay and the cartridge is supplied as a consumable.

Sample-to-answer microfluidic cartridge

Growing community of inventors

Mercer Island, WA, United States of America

Matthew Scott Bragd