Roche Nimblegen, Inc.

Roche Sequencing Solutions, Inc.

Roche Diagnostics Gmbh

F. Hoffmann-la Roche Ag

Matthew Rodesch

Thomas J Albert

Todd Richmond

Klaus-Peter Stengele

Tom Albert

Jeffrey Jeddeloh

Thomas Albert

Jochen Buehler

Markus Ott

Jochen Bühler

Jacob Kitzman

Mark D'Ascenzo

Christina Middle

Jeff Jeddeloh

Mark D'ASCENZO

The present invention provides methods and systems for the capture and enrichment of target nucleic acids and analysis of the enriched target nucleic acids. In particular, the present invention provides for the enrichment of targeted sequences in a solution based format.

Methods and systems for solution based sequence enrichment

An oligopeptide microarray and methods for the synthesis thereof are presented. Further presented is a microarray on a solid support comprising at least about 10,000 oligopeptide features per cmand preferably at least about 50,000 oligopeptide features per cm.

Methods for synthesis of an oligopeptide microarray

The present invention relates to a microarray comprising at least 50,000 oligopeptide features per cmwhere the oligopeptide features represent at least 50%, 60%, 70%, 80%, 90%, 95%, 99%, or 100% of the proteome of a virus or an organism. The present invention further relates to methods for the synthesis of such microarrays and methods of using microarrays comprising at least 50,000 oligopeptide features per cm. In an embodiment of the invention, the oligopeptide features represent proteins expressed in the same species, wherein the oligopeptide features are presented in a tiling pattern representing at least about 5,000 to-at least about 25,000 proteins expressed in a species. In some embodiments, the oligopeptide microarray features represent proteins expressed in the same species, wherein the microarray features are present in a tiling pattern that represents at least about 5,000 to at least about 50,000 expressed proteins.

Whole proteome tiling microarrays

The present invention provides methods and compositions for the enrichment of target nucleic acids in a microarray system. In particular, the present invention provides methods and compositions for uniform enrichment of target nucleic acid molecules in a microarray format. The present invention also provides for intentionally non-uniform enrichment among target nucleic acid molecules.

Methods and systems for uniform enrichment of genomic regions

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Stoughton, WI, United States of America

Matthew Rodesch