Streck, Inc.

Creighton University

Matthew R Kreifels

Scott E Whitney

Joel R TerMaat

John J Flanagan

Hendrik Viljoen

Michael M Tilleman

James Dowling

Gregg Wilder

Philip Hills

Jeremie Jackson

Nancy D Hanson

Joel Lechner

Stacey Morrow

Laura R Porter

Cathy Scheer

Christopher Connelly

Stephanie Cossette

Maria Torres-Gonzalez

David Lee Oliver

Rebecca Anne Reiss

Randy Fowler

Jason R Gerold

Troy M Just

Hendrik J Viljoen

Joseph William Brooks

Bardhyl Qendro

Paul Dye

Norman P Michaud, Jr

Jamee Thieman

Maria Torres

Philip Hill

John Flanagan

Chris Connelly

Assays and methods for detecting resistance to beta-lactam antibiotics including detection of multiple β-lactamase family specific gene targets by polymerase chain reaction or microarray. One or more kits including primers and/or probes for identification of β-lactamase genes selected from the group consisting of one or more of the following: MOX-like, FOX-like, ACC-like, ACT/MIR-like, CMY-2-like, DHA-like, CTX-M-14-like, CTX-M-15-like, VIM-like, NDM-like, IMP-like, KPC-like, and OXA-48-like, OXA-51-like, OXA-143-like, OXA-58-like, OXA-23-like, OXA-24/40-like, TEM-like, and SHV-like. A kit may also include one or more primers and/or probes for the identification a non-beta lactamase gene family which confers antibiotic resistance, such as the MCR-1 gene.

Assays and methods for determining microbial resistance

An improved device and system for facilitating polymerase chain reaction including a light source, detector, waveguide, and filters that occupy minimal space and facilitate detection of stationary samples, reduced sample read time, and simultaneous reading of multiple light wavelengths.

Devices for real-time polymerase chain reaction

Assays and methods for detecting resistance to beta-lactam antibiotics including detection of multiple &#x3b2;-lactamase family specific gene targets by polymerase chain reaction or microarray. One or more kits including primers and/or probes for identification of &#x3b2;-lactamase genes selected from the group consisting of one or more of the following: MOX-like, FOX-like, ACC-like, ACT/MIR-like, CMY-2-like, DHA-like, CTX-M-14-like, CTX-M-15-like, VIM-like, NDM-like, IMP-like, KPC-like, and OXA-48-like, OXA-51-like, OXA-143-like, OXA-58-like, OXA-23-like, OXA-24/40-like, TEM-like, and SHV-like. A kit may also include one or more primers and/or probes for the identification a non-beta lactamase gene family which confers antibiotic resistance, such as the MCR-1 gene.

An improved device and system for facilitating polymerase chain reaction including a light source, detector, waveguide, and filters that occupy minimal space and facilitate reduced sample read time and rapid reading of multiple light wavelengths.

Real-time optical system for polymerase chain reaction

A thermocycling device and method of operating a thermocycler instrument, the instrument including a sample holder, at least one thermal cycling element, and at least one first and second temperature sensors, for causing the sample holder containing the at least one sample to undergo polymerase chain reaction amplification by repeated cycling between at least a denaturation heating stage and an annealing cooling stage. The first temperature corresponding with the temperature of the sample holder is monitored using the at least one first temperature sensor, and a second temperature corresponding with the temperature external of the sample holder is monitored using the at least one second temperature sensor. Based upon the first temperature and the second temperature, the power that is delivered to the at least one thermal cycling element of the instrument is dynamically controlled.

Rapid thermocycler system for rapid amplification of nucleic acids and related methods

Laboratory analysis device housing

An improved tube including a closure portion, a strap integrally connected to the closure portion and being configured for defining a living hinge, a body portion having a longitudinal axis and an outer wall generally circumscribing the longitudinal axis, and being integrally and hingedly connected with the closure portion by way of the strap. The body portion including a sample portion being generally elongated along the longitudinal axis and being configured for elastic deformation along a portion of its length, including in a direction that is generally transverse to the longitudinal axis so that at least a portion of the wall structure compressively and resiliently deforms and engages a wall defining an opening in a sample block of a polymerase chain reaction amplification device, and the first outer wall dimension of the sample portion reduces to a smaller second outer wall dimension.

Sample tube having particular utility for nucleic acid amplification

Sample tube

Blood sample tube label

Growing community of inventors

Omaha, NE, United States of America

Matthew R Kreifels