Merck Sharp + Dohme Corp.

Other

Matthew P Watson

Michael Albert Winters

Patrick L Ahl

Patrick McHugh

Akhilesh Bhambhani

Jessica Sinacola

Emily Wen

Morrisa C Jones

Christopher Jon Farrell

Justin Stanbro

Daniel D Roth

Patrick Mchugh

Patrick Leonard Ahl

Jessica R Sinacola

A method is described for producing a pneumococcal capsular polysaccharide protein conjugate in which one or more activated pneumococcal polysaccharides of particular pneumococcal serotypes and carrier protein are separately lyophilized, the separately lyophilized polysaccharides and carrier protein are separately reconstituted in an organic solvent, and the reconstituted polysaccharide and carrier protein are then combined together by Tee-mixing and conjugated together to produce polysaccharide carrier protein conjugates. A plurality of conjugates, each comprising polysaccharides of a particular serotype, may be used to produce multivalent pneumococcal immunogenic compositions having a combination of conjugates for use in vaccines.

Methods for producingcapsular polysaccharide carrier protein conjugates

A method is described for producing a pneumococcal capsular polysaccharide protein conjugate in which one or more activated pneumococcal polysaccharides of particular pneumococcal serotypes and carrier protein are separately lyophilized in the form of lyospheres of carrier protein and lyospheres of the one or more polysaccharides. A predetermined amount of carrier protein lyospheres and activated polysaccharide lyospheres are mixed together and the mixture reconstituted in an organic solvent to produce polysaccharide carrier protein conjugates. A plurality of conjugates, each comprising polysaccharides of a particular serotype, may be used to produce multivalent pneumococcal immunogenic compositions having a combination of conjugates for use in vaccines.

Methods for producing streptococcus pneumoniae capsular polysaccharide carrier protein conjugates from lyospheres

A method is described for reconstituting lyophilized mutant diphtheria toxin in dimethyl sulfoxide for use in producing pneumococcal capsular polysaccharide mutant diphtheria toxin conjugates.

Methods for providing a homogenous solution of lyophilized mutant diptheria toxin in dimethylsulfoxide

A chemical formulation for cleaning and treating plastic and other surfaces comprises linseed oil in a concentration of 43%-59% wt.; mineral spirits in a concentration of 41%-57% wt.; and carnauba wax in a concentration of 2%-10% wt. A method of using the chemical formulation comprises applying it the composition to a surface.

Auto reconditioning solution

A beverage composition for weight loss is provided which includes cayenne pepper; a palate enhancing agent selected from the group consisting of fruits, herbs, vegetables and mixtures thereof, the agent including pulp of the fruits, the herbs, the vegetables or the mixtures thereof; a least two spices selected from the group consisting of black or white pepper, ginger, cinnamon, mustard seed and mixtures thereof; and water in an amount from 80 to 99% by weight of the composition; and wherein the composition has a total calorie content from 0 to 100 based on 100 gram of the composition.

Weight loss compositions and methods for appetite suppression

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Ottsville, PA, United States of America

Matthew P Watson