Northwestern University

Cornell University

Wisconsin Alumni Research Foundation

Glycobia, Inc.

Merritt, Judith H.

Valderrama-rinco, Juan D.

Fisher, Adam, C.

Delisa, Matthew P.

Hamilton, Brian S.

Matthew P Delisa

Michael Christopher Jewett

Jessica Carol Stark

Thapakorn Jaroentomeechai

Miguel A Dominguez

Charles H Haitjema

Judith H Merritt

Sydnor T Withers

Juan D Valderrama-Rincon

Thomas J Mansell

Adam C Fisher

Brian S Hamilton

Disclosed are components and systems for cell-free glycoprotein synthesis (CFGpS). In particular, the components and systems include and utilize prokaryotic cell lysates from engineered prokaryotic cell strains that have been engineered to enable cell-free synthesis of glycoproteins.

Cell-free glycoprotein synthesis (CFGPS) in prokaryotic cell lysates enriched with components for glycosylation

Disclosed are methods, systems, components, and compositions for cell-free synthesis of glycosylated proteins, which may be utilized in vaccines, including anti-bacterial vaccines. The glycosylated proteins may include a bacterial polysaccharide conjugated to a carrier, which may be utilized to generate an immune response in an immunized host against the polysaccharide conjugated to the carrier. Suitable carriers may include but are not limited toprotein D (PD),porin protein (PorA),toxin (CRM 197),toxin (TT), andmaltose binding protein, and variants thereof.

Bioconjugate vaccines' synthesis in prokaryotic cell lysates

Growing community of inventors

Ithaca, NY, United States of America

Matthew P Delisa