Surface Logix, Inc.

Synergy Innovations, Inc.

Matthew L Brown

Enoch Kim

Gregory Kirk

Emanuele Ostuni

Olivier Schueller

Paul Sweetnam

Bernardo D Aumond

Brian Benoit

Richard W Couch, Jr

Robert C Dean, Jr

David F Nelson

Mark W Blanchard

A method and apparatus for forming high-speed water droplets takes advantage on a synergy of features disclosed by the present invention including a low Weber number in a chamber in which a liquid stream breaks up into droplets; a reduced break up distance for forming the water droplets by introducing turbulence into the liquid source, thereby providing a unit or tool that is of practical size; accomplishing the generation of high-speed water droplets without using a complex driver; reducing the liquid's surface tension thereby facilitating the creating of near spherical droplets; and maintaining the droplets in form until they strike the surface of a workpiece.

Method and apparatus for forming high-speed liquid

The present invention discloses a device for monitoring haptotaxis including a housing defining a chamber. The chamber comprises: a first well region including at least one first well, the first well configured to receive a test agent therein and further including biomolecules immobilized therein; a second well region including at least one second well, the second well region configured to receive a sample comprising cells therein and further being horizontally offset with respect to the first well region in a test orientation of the device; and a channel region including at a least one channel connecting the first well region and the second well region with one another, the channel region further including biomolecules immobilized therein.

Device for monitoring haptotaxis

The present invention discloses a test device including a support member; a top member mounted to the support member, wherein in the support member and the top member are configured such that they together define a discrete chamber. The discrete chamber includes a first well region including at least one first well; a second well region including at least one second well; and a channel region including at least one channel connecting the first well region and the second well region with one another. The second region is preferably horizontally offset with respect to the first well region in a test orientation of the device.

Test device and method of making same

An image processing method for use in analyzing image data of a cellular migration assay. The method includes defining a major axis within the image data that is perpendicular to an orientation of channels in the image data, determining an aggregate light intensity within the image data along the major axis, and identifying locations of channels within the image data from the projection.

Image processing method for use in analyzing data of a chemotaxis or haptotaxis assay

The present invention discloses a device for monitoring chemotaxis or chemoinvasion including a housing comprising: a support member and a top member, the top member mounted to the support member by being placed in substantially fluid-tight conformal contact with the support member. The support member and the top member are configured such that they together define a discrete chamber adapted to allow a monitoring of chemotaxis or chemoinvasion therein. The discrete chamber includes a first well region including at least one first well, the at least one first well configured to received a test agent therein; a second well region including at least one second well, the at least one second well configured to receive a sample comprising cells therein; and a channel region including at least one channel connecting the first well region and the second well region with one another. The second well region is preferably horizontally offset with respect to the first well region in a test orientation of the device.

Cell motility and chemotaxis test device and methods of using same

The present invention discloses a method of monitoring haptotaxis comprising: providing a device for monitoring haptotaxis having a housing defining a chamber. The chamber includes: a first well region including at least one first well, the first well region configured to receive a test agent therein and further including biomolecules immobilized therein; a second well region including at least one second well, the second well region configured to receive a sample comprising cells therein and further being horizontally offset with respect to the first well region in a test orientation of the device; and a channel region with biomolecules immobilized therein and including at least one channel connecting the first well region and the second well region with one another. The method also includes forming a surface concentration gradient along a longitudinal axis of the chamber by decreasing the concentration of biomolecules from the at least one first well to the at least one second well; placing a first sample comprising cells in the at least one second well; and monitoring haptotaxis of the cells.

Method of monitoring haptotaxis

The present invention discloses a method of monitoring chemotaxis or chemoinvasion comprising: providing a device for monitoring chemotaxis. The device includes a support member; a top member mounted to the support member by being placed in substantially fluid-tight, conformal contact with the support member. The support member and the top member are configured such that they together define a discrete assay chamber. The discrete assay chamber includes a first well region including at least one first well, the at least one first well being configured to receive a soluble test substance therein; a second well region including at least one second well, the at least one second well being configured to receive a sample comprising cells therein; and a channel region including at least one channel connecting the first well region and the second well region. Preferably, at least one second well is horizontally offset with respect to the first well region in a test orientation of the device. The method further comprises placing the soluble test substance in the at least one first well; forming a solution concentration gradient along a longitudinal axis of the chamber; placing a first sample comprising cells in the at least one second well; and monitoring chemotaxis of the cells.

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Watertown, MA, United States of America

Matthew L Brown