Lonza Walkersville, Inc.

Octane Biotech Inc.

Matthew Hewitt

Eytan Abraham

Anandita Seth

Bingnan Gu

Young Shin

Caitlin M Guenther

Yaling Shi

Timothy Smith

Nuala Trainor

Kelly Purpura

Chase McRobie

Nicholas Ostrout

The present disclosure provides cell culture chambers for use in automated cell engineering systems, and in particular, cell culture chambers that include improved cell-contacting surfaces. Improved cell-contacting surfaces can include a surface coating that promotes cell growth, adherence, differentiation, maintenance of phenotype, and/or improves transduction; a cell-contacting surface comprising a non-porous, gas-permeable material; as well as other modifications to the cell-contacting surfaces. Cassettes comprising the cell culture chambers are also provided.

Cell culture chamber with improved cell-contacting surfaces

The present disclosure provides an automated method of producing viral vectors, utilizing engineered viral vector-producing cell lines within a fully-enclosed cell engineering system. Exemplary viral vectors that can be produced include lentivirus vectors, adeno-associated virus vectors, baculovirus vectors and retrovirus vectors.

Automated production of viral vectors

The present disclosure provides methods for assessing and optimizing cellular quality of a cell-based therapy that is being produced in an automated cell engineering system. The methods suitably include monitoring molecular characteristics of the cells before, during, and after the automated process to provide feedback to the process parameters. In embodiments, the cells being produced are Chimeric Antigen Receptor (CAR) T-cells.

Quality control methods for automated cell processing

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Walkersville, MD, United States of America

Matthew Hewitt