Boehringer Ingelheim International Gmbh

Genentech, Inc.

University of North Carolina at Chapel Hill

Other

Boehringer Ingelheim Gmbh

Genetech, Inc.

Matthew Cotten

Ernst Wagner

Max L Birnstiel

David T Curiel

Kurt Zatloukal

Berndt Oberhauser

Christian Plank

Walter G Schmidt

Susanna Chiocca

Hartmut Beug

Adam Baker

Gotthold Schaffner

Robert Kurzbauer

Anne-Isabelle Michou

Jolanta Glotzer

Walter G M Schmidt

Gerhard Christofori

Ping-chuan Hu

Amelia Compagni

Harald Kandolf

Recombinant CELO virus or CELO virus DNA with a deletion at the right end of the viral genome that allows insertion of large pieces of foreign DNA. The virus is useful as a vaccine for animals, in particular birds, and for gene therapy and vaccine applications in humans. The virus can also be used for recombinant protein production.

Recombinant celo virus and celo virus DNA

Genetics units for inhibiting the function of RNA

Recombinant CELO virus or CELO virus DNA with a deletion or disruption of the Gam1 gene, optionally combined with deletions at the right or left end of the viral genome that allow insertion of large pieces of foreign DNA. The virus is useful as a vaccine for animals, in particular birds, and for gene therapy and vaccine applications in humans. The virus can also be used for recombinant protein production.

Recombinant, replication defective CELO virus and CELO virus DNA

A CELO virus obtained by in vitro manipulation of a plasmid-cloned CELO virus DNA is suitable for the production of vectors for gene therapy and as a vaccine against infectious diseases in humans and animals, particularly birds.

Chicken embryo lethal (CELO) virus

Chicken embryo lethal orphan (CELO) virus

The toxicity problems which occur when foreign material is introduced into higher eukaryotic cells, particularly during transfection with DNA, are countered by causing gene products to be expressed in the cell, which block the apoptosis triggered by the transfection process, and/or by treating the cells with anti-inflammatory substances. Preferably, Bcl-2, E1B 19K or an anti-apoptotically active gene of chicken adenovirus CELO is used as the anti-apoptosis gene whilst the inflammatory substance used is adenovirus VA1, which is introduced into the cell in the form of VA1-DNA. Using these methods, long-lasting gene expression can be achieved.

Chicken embryo lethal orphan (CELO) virus GAM-1

A composition for the transfection of higher eucaryotic cells, comprising complexes of nucleic acid, a substance having an affinity for nucleic acid and optionally an internalizing factor, contains an endosomolytic agent, e.g. a virus or virus component, which may be conjugated. The endosomolytic agent, which is optionally part of the nucleic acid complex, is internalized into the cells together with the complex and releases the contents of the endosomes into the cytoplasm, thereby increasing the gene transfer capacity. Pharmaceutical preparations, transfection kits and methods for introducing nucleic acid into higher eucaryotic cells by treating the cells with the composition are also disclosed.

Composition for introducing nucleic acid complexes into higher eucaryotic cells

Composition for introducing nucleic acid complexes into higher

Composition comprising an endosomolytic agent for introducing nucleic

Complexes containing nucleic acid which can be taken-up by endocytosis

The invention relates to a system for transporting nucleic acids into the cell, which is effected by receptor-mediated endocytosis. Using a transferrin-polycation conjugate, a complex can be formed with the polyanionic nucleic acid. This complex is bound to the transferrin receptor, which is highly regulated in growing cells, and absorbed into the cell. Suitable nucleic acids include those which inhibit specific genes or the RNA function, such as antisense oligonucleotides or ribozymes or the genes coding for them. The invention further relates to a process for introducing nucleic acids into the cells, transferrin-polycation/nucleic acid complexes and pharmaceutical preparations containing them.

Protein-polycation nucleic acid complexes and methods of use

Endosomolytically active particles

Adenovirus for delivering foreign DNA into higher eukaryotic cells

Growing community of inventors

Vienna, Austria

Matthew Cotten