Trustees of the Colorado School of Mines

Alliance for Sustainable Energy, LLC

Matthew C Posewitz

Robert Jinkerson

Randor Radakovits

Jonathan E Meuser

Michael Seibert

Maria Lucia Ghirardi

Devin Karns

Paul W King

Sharon L Smolinski

Victoria H Work

Edward Dempsey

Methods, processes, and systems for the production of lipids and starch from modified algae are disclosed. In one embodiment, the modified algae over-expresses isoamylase and accumulates much higher amounts of starch than unmodified algae. In some embodiments, the modified algae comprises one or more copies of an isoamylase expression construct. In one embodiment, the modified algae is a sta7mutant with a starchless phenotype that has been complemented with one or more copies of the wild-type genomic STA7 isoamylase gene construct. The complemented, modified algae accumulates much greater amount of starch than an unmodified algae and may be used to produce large amounts of starch and/or lipids.

Modified algae for improved biofuel productivity

A process for expression of active [FeFe]-hydrogenase in a host organism that does not contain either the structural gene(s) for [FeFe]-hydrogenases and/or homologues for the maturation genes HydE, HydF and HyG, comprising: cloning the structural hydrogenase gene(s) and/or the maturation genes HydE, HydF and HydG from an organisms that contains these genes into expression plasmids; transferring the plasmids into an organism that lacks a native [FeFe]-hydrogenase or that has a disrupted [FeFe]-hydrogenase and culturing it aerobically; and inducing anaerobiosis to provide [FeFe] hydrogenase biosynthesis and H?2#191 production.

Process and genes for expression and overexpression of active [FeFe] hydrogenases

The present disclosure is directed to novel polynucleotide sequences for use in. The novel polynucleotide sequences include control sequences and coding sequences. Also disclosed are novel gene expression constructs whereinpromoters/control regions are operatively linked toor non-coding sequences. These novel polynucleotide sequences and expression constructs can be introduced intoand can recombine into thegenome. Expression from these polynucleotide sequences and expression constructs can enhancebiomass and/or lipid biosynthesis. Also disclosed are methods for modifying, for example by stably transformingwith nucleic acid sequences, growing the modified, and obtaining biomass and biofuels from the modified

[object Object]

A system for measuring hydrogen (H) and oxygen (O) including a sample system, a signal processing and system control device, at least one polarograph device and a computing device equipped with software for environmental and biological data analysis incorporating both user-defined and environmental data acquired by the signal processing and system control device. The system measures gas level or exchange (production and/or consumption) in the field or laboratory. In some embodiments, the system uses two Clark-type polarograph devices oppositely polarized with gas-specific polarographic circuits, wherein one polarograph device measures Hgas and the other measures Ogas. In some embodiments, the sample system includes an environmentally-controlled sample housing for accepting the polarograph devices and holding a test sample in a reservoir. The system measures Hor Oexchange in microbial samples, including microbial phototrophs which couple light harvesting to Hand/or Oexchange.

Polarographic instrument system for oxygen and hydrogen measurement

Growing community of inventors

Golden, CO, United States of America

Matthew C Posewitz