Maxygen, Inc.

Codexis Mayflower Holdings, LLC

Codexis, Inc.

Other

Codexis Mayflower Holding, LLC

Matthew B Tobin

Jeremy S Minshull

Phillip A Patten

Willem P C Stemmer

Jon Edward Ness

Claes Gustafsson

Stephen B Del Cardayre

Lorraine Joan Giver

Linda A Castle

Sergey A Selifonov

Claus M Krebber

Steven Henry Bass

Joseph A Affholter

Venkiteswatan Mani Subramanian

William P C Stemmer

Stephen delCardayre

Stephen DelCardayre

Gjalt W Huisman

Steve Bass

Ying-Xin Zhang

Ling Yuan

Anthony Cox

Brian S Carr

Mark D Welch

Robin A Emig

Andreas Crameri

Sun Ai Raillard

Venkiteswaran Subramanian

Stanley Goldman

Pascal Longchamp

Emily Mundorff

Ish Kumar Dhawan

Richard John Fox

Stephane J Jenne

S Christopher Davis

Stephan Jenne

Birthe Borup

Ranjini Chatterjee

Simon Christopher Davis

Svetlana Balatskaya

Kenneth W Mitchell

Tony Cox

Les Partridge

Claus Gustafsson

Willem Pc Stemmer

The invention provides methods employing iterative cycles of recombination and selection/screening for evolution of whole cells and organisms toward acquisition of desired properties. Examples of such properties include enhanced recombinogenicity, genome copy number, and capacity for expression and/or secretion of proteins and secondary metabolites.

Evolution of whole cells and organisms by recursive sequence recombination

The present disclosure relates generally to polypeptides having improved alanine 2,3-aminomutase (AAM) activity, the polynucleotides encoding the AAM polypeptides, and expression vectors and host cells for expressing the AAM polypeptides.

Alanine 2,3-aminomutases and related polynucleotides

Integrated systems and methods for diversity generation and screening are provided. The systems use common fluid and array handling components to provide nucleic acid diversification, transcription, translation, product screening and subsequent diversification reactions.

Integrated systems and methods for diversity generation and screening

In silico nucleic acid recombination methods, related integrated systems utilizing genetic operators and libraries made by in silico shuffling methods are provided. One disclosed method involves identifying and producing a polypeptide. The method may involve (a) receiving data identifying sequences of two or more parental polypeptides or parental nucleic acids that encode the polypeptides; (b) selecting one or more cross-over sites on the sequences thereby defining one or more recombinant polypeptides or recombinant nucleic acids; (c) selecting at least one of the recombinant polypeptides or recombinant nucleic acids; (d) recombining one or more oligonucleotides, at least one of which has a sequence matching some or all of that of the recombinant polypeptides or recombinant nucleic acids selected in (c); (e) selecting at least some of the recombined oligonucleotides produced in (d); and (f) producing a polypeptide encoded by the selected nucleic acid.

Methods for identifying and producing polypeptides

In silico nucleic acid recombination methods, related integrated systems utilizing genetic operators are used to identify sets of oligonucleotides for in vitro recombination. In certain embodiments, the methods involve inputting amino acid sequence character strings into a digital system and back-translating the amino acid character strings into a plurality of nucleic acid character strings. The nucleic acid character strings are then processed to define and select at least one recombinant nucleic acid for use in identifying one or more oligonucleotides for in vitro recombination.

Methods for identifying sets of oligonucleotides for use in an in vitro recombination procedures

In silico nucleic acid recombination methods, related integrated systems utilizing genetic operators and libraries made by in silico shuffling methods are provided.

Method for making polynucleotides having desired characteristics

In silico nucleic acid recombination methods, related integrated systems utilizing genetic operators are used to identify sets of oligonucleotides for in vitro recombination.

Methods for identifying sets of oligonucleotides for use in an in vitro recombination procedure

Integrated system for diversity generation and screening

'In silico' nucleic acid recombination methods, related integrated systems utilizing genetic operators and libraries made by in silico shuffling methods are provided.

Identifying oligonucleotides for in vitro recombination

The invention provides methods employing iterative cycles of recombination and selection/screening for evolution of whole cells and organisms toward acquisition of desired properties Examples of such properties include enhanced recombinogenicity, genome copy number, and capacity for expression and/or secretion of proteins and secondary metabolites.

Methods for making character strings, polynucleotides and polypeptides having desired characteristics

Growing community of inventors

San Carlos, CA, United States of America

Matthew B Tobin