Promega Corporation

Life Technologies Corporation

Matthew B Robers

Brock Binkowski

Keith V Wood

Lance P Encell

Mary P Hall

Monika G Wood

Thomas Machleidt

Thomas A Kirkland

Poncho Meisenheimer

Melanie Dart

James Unch

Nidhi Nath

Said A Goueli

Andrew S Dixon

Marie Schwinn

Hicham Zegzouti

Subhanjan Mondal

Dileep K Pulukkunat

Michael R Slater

Ce Shi

Wenhui Zhou

Joel R Walker

Cesear Corona

Mei Cong

James Vasta

Dieter Klaubert

Chad Zimprich

Michael P Valley

Kurt William Vogel

Sarah Duellman

Roderick G Flemming

Steven Riddle

Kristin Huwiler

Robert Horton

Gregory Michaud

Kevin Vedvik

Brock F Binkowski

An isolated polynucleotide encoding a modified luciferase polypeptide and substrates. The OgLuc variant polypeptide has at least 60% amino acid sequence identity to SEQ ID NO: 1 and at least one amino acid substitution at a position corresponding to an amino acid in SEQ ID NO: 1. The OgLuc variant polypeptide has at least one of enhanced luminescence, enhanced signal stability, and enhanced protein stability relative to the corresponding polypeptide of the wild-typeluciferase.

-derived luciferases, novel coelenterazine substrates, and methods of use

Provided herein are compositions and methods for the assembly of a bioluminescent complex from two or more non-luminescent (e.g., substantially non-luminescent) peptide and/or polypeptide units. In particular, bioluminescent activity is conferred upon a non-luminescent polypeptide via structural complementation with another, complementary non-luminescent peptide.

Activation of bioluminescence by structural complementation

An isolated polynucleotide encoding a modified luciferase polypeptide and substrates. The OgLuc variant polypeptide has at least 60% amino acid sequence identity to SEQ ID NO: 1 and at least one amino acid substitution at a position corresponding to an amino acid in SEQ ID NO: 1. The OgLuc variant polypeptide has at least one of enhanced luminescence, enhanced signal stability, and enhanced protein stability relative to the corresponding polypeptide of the wild-type

Oplophorus-derived luciferases, novel coelenterazine substrates, and methods of use

Provided herein are compounds that are broad-spectrum protein kinase binding agents, detectable tracers comprising such compounds, and method of use thereof for the detection of protein kinases.

Broad spectrum kinase binding agents

Provided herein are systems and methods for characterizing target/ligand engagement. In particular, luciferase-labeled polypeptide targets are used to detect or quantify target/ligand engagement (e.g., within a cell or cell lysate).

Luciferase-based thermal shift assays

Activation of bioluminescene by structural complementation

Compounds that may inhibit-derived luciferases are disclosed as well as compositions and kits comprising the compounds, and methods of using the compounds.

Functionalized NANOLUC inhibitors

Growing community of inventors

Madison, WI, United States of America

Matthew B Robers