Lawrence Livermore National Security, LLC

University of California

United States of America As Represented by the Administrator of Nasa

Matthew A Coleman

Wei He

Kit S Lam

Juntao Luo

Paul Henderson

Brent W Segelke

Feliza A Bourguet

Nicholas O Fischer

Craig D Blanchette

Amy Rasley

Brian E Souza

Paul J Jackson

Stephen M Lane

Dennis L Matthews

Todd Peterson

Dan McFarland Park

Sergio E Wong

Howard Harris

Yongqin Jiao

Patrik D'haeseleer

Mimi-Cho Yung

Kenneth W Overton

William J Benett

Robin R Miles

Jing Li

Peter T Beernink

Paul D Hoeprich

Cristina E Davis

Allen T Christian

Shanavaz L Nasarabadi

R Holland Cheng

Lawrence Dugan

David J Loftus

Li Xing

Mimi Cho Yung

Martina Felderman

James D Tucker

Timothy S Carpenter

Joanna Albala

Angela Clare Evans

Tore Straume

Anup K Singh

Rupa S Rao

Deanna Lynn Thompson

Sebastian Wachsmann-Hogiu

Francesca S Pearson

Kurt I Kamrud

Bolyn Hubby

Heather D Gouvis

Nathaniel S Wang

The present invention provides a nanodisc with a membrane scaffold protein. The nanodisc includes a membrane scaffold protein, a telodendrimer and a lipid. The membrane scaffold protein can be apolipoprotein. The telodendrimer has the general formula PEG-L-D-(R), wherein D is a dendritic polymer; L is a bond or a linker linked to the focal point group of the dendritic polymer; each PEG is a poly(ethylene glycol) polymer; each R is and end group of the dendritic polymer, or and end group with a covalently bound hydrophobic group, hydrophilic group, amphiphilic compound, or drug; and subscript n is an integer from 2 to 20. Cell free methods of making the nanodiscs are also provided.

Apolipoprotein nanodiscs with telodendrimer

Nanolipoprotein particles comprising at least a scaffold protein component and a membrane lipid component and related complexes, compositions, methods and systems are described, in which the membrane lipid component comprises at least one or more membrane forming lipids and one or more cationic lipids.

Nanolipoprotein particles and related compositions methods and systems for loading RNA

A telodendrimer-nanolipoprotein particle (t-NLP), comprising one or more membrane forming lipids, one or more telodendrimers, and a scaffold protein and amajor outer membrane protein (MOMP) comprising a MOMP hydrophobic region, and related compositions methods and systems.

MOMP telonanoparticles, and related compositions, methods and systems

Targeted antimicrobials are described and related, compositions, methods and systems.

Targeted antimicrobials and related compositions, methods and systems

The present invention provides a nanodisc with a membrane scaffold protein. The nanodisc includes a membrane scaffold protein, a telodendrimer and a lipid. The membrane scaffold protein can be apolipoprotein. The telodendrimer has the general formula PEG-L-D-(R), wherein D is a dendritic polymer; L is a bond or a linker linked to the focal point group of the dendritic polymer; each PEG is a poly(ethylene glycol) polymer, each R is and end group of the dendritic polymer, or and end group with a covalently bound hydrophobic group, hydrophilic group, amphiphilic compound, or drug; and subscript n is an integer from 2 to 20. Cell free methods of making the nanodiscs are also provided.

This disclosure provides microbes engineered to detect virulent and spore states of pathogens and release an appropriate therapeutic response accordingly and compositions and methods of use of the same.

Dynamically-adaptive live therapeutic agents and methods of use thereof

Provided herein are methods and systems for the production of a nanolipoprotein particle (NLP) that includes a scaffold protein a membrane forming lipid and optionally a target protein. At least one of the scaffold protein and target protein can be provided through an IVT system. The membrane forming lipid, scaffold protein and optionally the target protein can be assembled for a time and under conditions that allow obtaining high yield NLPs, NPLs with an increased solubility, an NLP of a controlled size, and/or an NLP having a size predetermined to include a pre-selected target protein.

Methods and systems for producing nanolipoprotein particles

The present invention provides a nanodisc with a membrane scaffold protein. The nanodisc includes a membrane scaffold protein, a telodendrimer and a lipid. The membrane scaffold protein can be apolipoprotein. The telodendrimer has the general formula PEG-L-D-(R), wherein D is a dendritic polymer; L is a bond or a linker linked to the focal point group of the dendritic polymer; each PEG is a polyethylene glycol) polymer; each R is and end group of the dendritic polymer, or and end group with a covalently bound hydrophobic group, hydrophilic group, amphiphilic compound, or drug; and subscript n is an integer from 2 to 20. Cell free methods of making the nanodiscs are also provided.

The invention described herein relates to methods, systems, compositions and cells to provide one or more toxic non-native proteins in a cell, wherein the one or more toxic non-native proteins are contained in at least one empty microcompartment within the cell.

Methods and systems for engineering bacterial systems to shield toxicity during non-native protein expression and purification

A system that integrates several technologies into a single, portable medical diagnostic apparatus for analyzing a sample body fluid (liquid and/or gas): (1) a mechanism to capture airborne microdroplets and to separate the body fluid into a first fluid component (primarily gas) and a second fluid component (primarily liquid); (2) a volatilizer to convert a portion of the second fluid component into a third fluid component that is primarily a gas; (3) a functionalized nanostructure (NS) array configured to receive, identify, and estimate concentration of at least one constituent in the first and/or third fluid components; (4) a miniaturized differential mobility spectrometer (DMS) module; and (5) a biomarker sensor, to detect volatile and non-volatile molecules in a sample fluid, which may contain one or more components of blood, breath, perspiration, saliva, and urine.

Portable medical diagnosis instrument

A device and array coupled to capture molecules are provided. Specifically, the device and array can be used for detecting the presence and concentration of biomarkers in a sample from a subject. The device and array can also allow the use of a method for scoring a sample for, e.g., the purpose of diagnosing a disease. The method can also be advantageous to applications where there is a need to accurately determine the disease stage of a subject for the purpose of making therapeutic decisions.

Device, array, and methods for disease detection and analysis

Growing community of inventors

Oakland, CA, United States of America

Matthew A Coleman