E.i. Dupont De Nemours and Company

Mattheos Koffas

James M Odom

Kelley C Norton

Rick Weizhang Ye

Andreas Schenzle

Qiong Cheng

Stephen K Picataggio

Pierre E Rouviere

Jean-Francois Tomb

Siqun Wang

Patricia C Brzostowicz

Tao Wang

Deana J Dicosimo

Deana DiCosimo

Edward S Miller

Genes have been isolated from16a sp. encoding the isoprenoid biosynthetic pathway. The genes and gene products are the first isolated from astrain that is capable of utilizing single carbon (C1) substrates as energy sources. The genes and gene products of the present invention may be used in a variety of ways for the production of isoprenoid compounds in a variety of organisms.

Genes involved in isoprenoid compound production

A method for the production of carotenoid compounds is disclosed. The method relies on the use of microorganisms which metabolize single carbon substrates for the production of carotenoid compounds in high yields.

Carotenoid production from a single carbon substrate

A high growth methanotrophic bacterial strain capable of growth on a C1 carbon substrate has been isolated and characterized. The strain has the unique ability to utilize both methane and methanol as a sole carbon source and has been demonstrated to possess a functional Embden-Meyerhof carbon flux pathway. The possession of this pathway conveys an energetic advantage to the strain, making it particularly suitable as a production platform for the production of biomass from a C1 carbon source.

Production of feed using a denitrifying methanotrophic bacterial strain

Genes have been isolated from asp encoding enzymes in the carbon flux pathway. The genes encode a 2-keto-3-deoxy-6-phosphogluconate (KDPGA) and a fructose bisphosphate aldolase (FFBPA), as well as numerous other genes. The genes will be useful in C1 metabolizing microorganisms for the manipulation of the carbon flux pathway.

Methanotrophic carbon metabolism pathway genes and enzymes

A methanotrophic bacterium has been genetically engineered to produce cyclic terpenoids from geranyl pyrophosphate.

Production of cyclic terpenoids

Genes have been isolated from a Methylomonas sp encoding enzymes in the carbon flux pathway. The genes encode a 2-keto-3-deoxy-6-phosphogluconate (KDPGA) and a fructose bisphosphate aldolase (FFBPA), as well as numerous other genes. The genes will be useful in C1 metabolizing microorganisms for the manipulation of the carbon flux pathway.

High growth methanotropic bacterial strain

Genes have been isolated from Methylomonas 16 sp. encoding the isoprenoid biosynthetic pathway. The genes and gene products are the first isolated from a Methylomonas strain that is capable of utilizing single carbon (C1) substrates as energy sources. The genes and gene products of the present invention may be used in a variety of ways for the production of isoprenoid compounds in a variety of organisms.

Genes have been isolated from a Methylomonas sp encoding elements of the exopolysaccharide biosynthetic pathway. The genes and gene products are the first isolated from an organisms capable of utilizing single carbon (C1) substrates as energy sources. The genes are useful for engineering other C1 utilizing microorganisms to make altered levels of exopolysaccharide which is used in a variety of commercial applications.

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Wilmington, DE, United States of America

Mattheos Koffas