The University of Queensland

Seattle Children's Hospital

Seattle Biomedical Research Institute

Matt Trau

Gerard A Cangelosi

Jose Ramon Botella

Sakandar Rauf

Viktor Stein

Kirill Alexandrov

Eugene Wee

Ramanathan Pudhukode Vaidyanathan

Laura Garcia Carrascosa

Muhammad Johirul Alam Shiddiky

Yadveer Grewal

Muhammad J A Shiddiky

Abu Sina

Muhammad Johirul Shiddiky

Muhammad JA Shiddiky

Disclosed is an epigenetic biomarker that comprises clustered methylated genomic DNA which can self-assemble to form complexes that have distinct physicochemical properties relative to genomic DNA that lacks such clusters. Also disclosed are methods, systems, compositions and kits that takes advantage of these physicochemical properties for detecting clustered methylated genomic DNA including for determining likelihood of the presence of cancer.

Epigenetic biomarker and uses therefor

The present disclosure relates to biofragment compositions that comprise bioparticle fragments and at least one heterologous antigen-binding molecule. In some embodiments, the biofragment is typically derived from a larger, intact bioparticle that express the at least one heterologous antigen-binding molecule at the surface, and the biofragment has increased solubility to facilitate assays for antigen detection. The disclosure also relates the related methods of using and making the biofragment compositions, as well as systems and devices implementing the biofragment compositions. In some embodiments, the related methods, systems and devices do not require additional detection reagents, such as animal derived detection antibodies.

Cell-free biofragment compositions and related systems, devices, and methods

A method and kit for detecting a target nucleic acid is provided wherein combining an amplified nucleic acid with a particle such as a paramagnetic bead form a flocculated complex which can be detected by visual inspection. The volume of nucleic acid sample that can be detected is as low as a few microlitres. The method can be applied to in-the-field or point-of-care diagnosis for a rapid determination of the presence or absence of the target nucleic acid. The methylation status of a target nucleic acid can also be determined. The method and lit may have general applicability to detecting diseases in plants and animals, environmental testing and testing for contamination of foods and other edible products.

Nucleic acid detection method and kit

A device for the detection of target entities, the device including: a fluidic channel to conduct a liquid sample containing at least one type of target entity to be detected; and mutually spaced electrodes disposed along the fluidic channel; wherein the mutually spaced electrodes are functionalized to selectively bond to the at least one type of target entity and are configured to electro-hydrodynamically pump the liquid sample along the fluidic channel on application of a signal to the electrodes, such that selectivity of attachment of the at least one type of target entity to the electrodes is determined by at least one parameter of the signal.

Device and method for the detection of target entities

A biosensor molecule comprises: a protease amino acid sequence; at least one sensor comprising at least one sensor amino acid sequence which is responsive to at least one target molecule; and an inhibitor of the protease activity of said protease amino acid sequence; wherein the biosensor is switchable from a protease active to a protease inactive state, or from a protease inactive to a protease active state when said sensor responds to said target molecule. The biosensor protease may be a protease of a virus such as a Potyvirus or a Flavivirus wherein the inhibitor is an autoinhibitory peptide derived from the virus. The biosensor may respond to the target molecule allosterically or may be cleaved by a target protease molecule.

Protease-based biosensor

Growing community of inventors

Balmoral, Australia

Matt Trau