Octapharma Ag

Mats Jernberg

Stefan Winge

Gustav Gilljam

Andrea Neisser-Svae

Carin Borgvall

Ulrika Ericsson

A process for isolation and purification of a target protein by chromatography wherein the chromatography removes or depletes prions (PrP), comprising the steps of contacting a potentially PrPcontaminated sample comprising a target protein with a multimodal chromatographic material; setting buffer conditions so that the target protein is bound to the multimodal chromatographic material and whereas PrPis not binding to the multimodal chromatographic material; followed by elution of the target protein. a process for isolation and purification of a target protein free of prion protein (prp).

[object Object]

A process of purifying or enriching coagulation FVIII employing chromatography comprising the steps of providing a fraction containing FVIII in an aqueous solution having a high ionic strength; contacting the fraction containing FVIII with a multimodal resin; optionally washing the multimodal resin having FVIII adsorbed with an aqueous washing buffer; eluting FVIII containing fractions by an aqueous elution buffer comprising at least one amino acid which is positively charged at pH 6 to 8; and optionally collecting FVIII containing fractions in purified or enriched form.

Growing community of inventors

Stockholm, Sweden

Mats Jernberg