Gyros Patent Ab

Mats Inganas

Per X Andersson

Gunnar Kylberg

Helene Derand

Magnus Gustafsson

Gunnar Thorsen

Susanna Lindman

Bo Ek

Helene Dérand

Gunnar Thorsén

Johan Engstrom

Rickard Kange

Ann-Kristine Honerud

A microscale method for the characterization of one or more reaction variables that influence the formation or dissociation of an affinity complex comprising a ligand and a binder, which have mutual affinity for each other. The method is characterized in comprising the steps of: (i) providing a microfluidic device comprising a microchannel structures that are under a common flow control, each microchannel structure comprising a reaction microactivity; (ii) performing essentially in parallel an experiment in each of two or more of the plurality of microchannel structures, the experiment in these two or more microchannel structures comprising either a) formation of an immobilized form of the complex and retaining under flow conditions said form within the reaction microactivity, or b) dissociating, preferably under flow condition, an immobilized form of the complex which has been included in the microfluidic device provided in step (i), at least one reaction variable varies or is uncharacterized for said two or more microchannel structures while the remaining reaction variables are kept essentially constant; (iii) measuring the presentation of the complex in said reaction microactivity in said two or more microchannel structures; and (iv) characterizing said one or more reaction variables based on the values for presentation obtained in step (iii).

Characterization of reaction variables

A microfluidic device comprising one, two or more microchannel structures (-), each of which comprises a reaction microcavity (-) intended for retaining a solid phase material in the form of a wet porous bed. Each of said one, two or more microchannel structures comprises the solid phase material in a dry state together with a bed-preserving agent comprising one or more compounds having bed-preserving activity.

Preloaded microfluidic devices

A method for transporting in a microfluidic conduit an aliquot of a liquid, possibly containing a substance I, which is dissolved in the liquid and typically exhibits charged groups and/or hydrophobic groups. The method is characterized in that the liquid contains an amphiphilic macromolecular substance.

Protecting agent

Method for determining the amount of an analyte with a disc-shaped microfluidic device

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Uppsala, Sweden

Mats Inganas