University of California

University of Oregon

Aurora Biosciences Corporation

Mats F Ormo

Roger Y Tsien

Andrew B Cubitt

Roger Heim

S James Remington

Engineered fluorescent proteins, nucleic acids encoding them and methods of use.

Long wavelength engineered fluorescent proteins

This invention provides functional engineered fluorescent proteins with varied fluorescence characteristics that can be easily distinguished from currently existing green and blue fluorescent proteins. In one aspect, the invention provides nucleic acids, expression vectors and recombinant host cells comprising nucleotide sequences encoding functional engineered fluorescent proteins comprising aromatic substitutions at position 66 and a folding mutation. In one embodiment the invention provides for fluorescent proteins containing an aromatic substitution at Thr 203.

This invention provides functional engineered fluorescent proteins with varied fluorescence characteristics that can be easily distinguished from currently existing green and blue fluorescent proteins. In one embodiment the invention provides for the three dimensional structure and atomic coordinates of an Aequorea green fluorescent protein and methods for their use. In one embodiment, this invention provides a computational method of modeling the three dimensional structure of any other fluorescent protein based on the three dimensional structure of an Aequorea green fluorescent protein.

Growing community of inventors

Huddinge, Sweden

Mats F Ormo