Amersham Biosciences Ab

Ge Healthcare Bio-sciences Ab

Pharmacia Biotech Ab

Amersham Pharmacia Biotech Ab

Amersham Pharmacia Biotech Aktiebolag

Amersham Biociences Ab

Mats Carlsson

Hans Berg

Jan-Gunnar Gustafsson

Per Hedman

Annika Lindgren

J&ouml;rgen L&ouml;nngren

Per-&angst;ke Pernemalm

Anders Larsson

Philippe Busson

Per-Ake Pernemalm

Jorgen Lonngren

The present invention is a method of producing a chromatographic separation matrix, wherein porous polymeric particles comprised of two layers with different properties are prepared in a two-phase system by (a) providing porous polymeric particles with reactive groups on their surfaces; (b) washing said particles with a first solvent and draining the particles to enclose a first phase; (c) wetting the outer layer of the particles by adding a second solvent, which is essentially insoluble in the first solvent; (d) reacting the reactive groups in the outer layer by adding a reagent, which is essentially non-reactive in the first solvent; and (e) coupling of chromatographic binding groups to the reactive groups in the inner layer. The invention also encompasses a porous polymer particle suitable for use as a chromatographic separation matrix and a process of separation, wherein a matrix according to the invention is used.

Chromatographic two-layer particles

The present invention is a method of producing porous beads, which comprises the steps of providing a first liquid phase comprising a bead matrix material and essentially edgy templating particle(s), said particle(s) being treated with a surface modifying agent; providing a second liquid phase which is immiscible with the first liquid phase; contacting the first phase and the second phase under conditions resulting in an emulsion of droplets comprised of the first liquid phase dispersed in the continuous second liquid phase; transforming the droplets to mesoporous beads by solidification of the liquid; and removing the templating particle(s) from the beads without causing any essential change of the surrounding bead, whereby hierarchical networks of pores are provided in the beads.

Method of producing hierarchical porous beads

A method for the manufacture of a solid porous separation material based on a polysaccharide, said method comprising the steps of: (a) providing an aqueous solution (I) of a polysaccharide, (b) solidifying the solution, preferably by transforming the solution to a gel, and (c) optionally crosslinking the polysaccharide, with the proviso that, if step (c) is present, steps (b) and (c) may be carried out simultaneously. The method is characterized in that the polysaccharide provided in step (a) is modified by being inter-molecularly crosslinked to an extent such that the viscosity of the solution (I) is at least 110%, preferably at least 200%, of the viscosity of an aqueous solution (II) of the corresponding polysaccharide which has not been intermolecularly crosslinked and which is present in the same concentration as the inter-molecularly crosslinked polysaccharide is in solution (I)

Manufacturing process for porous material

A method for recovering a desired component from a sample solution with beads under stabilized fluidized bed conditions is provided. Also provided are support beads for use in downstream processing which comprise a polymer matrix into which glass or silica particles have been incorporated.

Process and means for down stream processing

A matrix including: a) a polymeric base matrix including macropores (pore system 1) and b) an interior material, possibly porous (pore system 2), retained within the macropores. The matrix is characterized in that there is a continuous free volume between the interior material and the pore walls of the macropores. A method for manufacturing a matrix including a base matrix having macropores in which an interior material is located. The method is characterized in including the steps: (I) providing a base matrix having macropores; (ii) filling the macropores with a soluble form of the interior material; (iii) transforming the insoluble from to an insoluble form; (iv) shrinking the insoluble form; and (v) irreversibly stabilizing the material in its shrunken form. The matrix can be used in separation methods, cell culturing, solid phase synthesis of organic molecules, and in catalytic reactions (such as enzyme reactions) and other uses in which porous support matrices are used.

Foamed material filled with inner material

The present invention relates to a method for producing porous polysaccharide beads, comprising: (a) mixing a water-based solution of a polysaccharide under stirring with an essentially water-immiscible first organic phase to form an emulsion which separates into two continuous phases; (b) adding a second organic phase comprising an emulsifier and an organic solvent to form a three-phase system; (c) allowing the three-phase system to emulsify; and (d) decreasing the temperature below the gelling point of the polysaccharide, thereby obtaining beads having two sets of pores, said method being characterised in that the emulsifier is a water-insoluble polymer capable of stabilizing said three-phase system.

Process for the production of polysaccharide beads

Support beads for downstream processing including a polymer, esp. a polysaccharide, matrix into which glass or silica particles, preferably quartz particles, have been incorporated, and their use in downstream processing, especially stabilized fluidized bed separations.

Support beads for downstream processing comprising a polymer, esp. a polysaccharide, matrix into which glass or silica particles, preferably quartz particles, have been incorporated, and their use in downstream processing, especially stabilized fluidized bed separations.

Growing community of inventors

Uppsala, Sweden

Mats Carlsson