Centre National De La Recherche Scientifique

Université Pierre Et Marie Curie (paris 6)

Ecole Normale Superieure

Fonds De L'espci-georges Charpak

Thales

Universite Bordeaux Segalen

Univerité Bordeaux Segalen

Sorbonne Université

Paris Sciences Et Lettres

Mathieu Morel

Vincent Studer

Denis Bartolo

Maxime Dahan

Jean-Christophe Galas

Renaud Dubois

Paul Gompel

Damien Baigl

Jacopo Vialetto

Sergii Rudiuk

The invention relates to a microfluidic system for controlling a concentration profile of molecules capable of stimulating a target, for example formed by an assembly of living cells, this system comprising: &#x2014;a microfluidic device () comprising at least one microfluidic channel () equipped with at least one inlet orifice () and with at least one outlet orifice () for at least one fluid; &#x2014;at least one means for supplying the microfluidic channel () with at least one fluid comprising molecules capable of stimulating the target; &#x2014;at least one chamber () or another microfluidic channel comprising a base () intended to receive the target; and &#x2014;at least one microporous membrane () separating the chamber () or the other microfluidic channel from the microfluidic channel (), said microporous membrane () being positioned away from the base () so that when the supply means provides the microfludic channel () with said at least one fluid flowing in laminar flow in contact with the microporous membrane (), the molecules capable of stimulating the target then diffuse, after having passed through the microporous membrane () through the chamber () or said other microfluidic channel in order to finally form a stable concentration profile in this chamber () or this other microfluidic channel.

Microfluidic system for controlling a concentration profile of molecules capable of stimulating a target

The invention relates to a microfluidic system for controlling a card for the concentration of molecules capable of stimulating a target, for example formed by an assembly of living cells, characterized in that the system comprises a microfluidic device () comprising: n&#x2267;1 microfluidic channel(s) (), the or each channel being provided with at least one inlet orifice for at least one fluid and with at least one outlet orifice for this fluid; n&#x2267;2 openings () formed in the microfluidic channel or distributed in the various microfluidic channels, said openings being arranged in one and the same plane so that they form a network having at least one dimension in this plane, the numbers n of microfluidic channel(s) and n of openings being linked by the relationship (I) with 1&#x2266;i&#x2266;n and n the number of openings for the channel c; at least one microporous membrane () covering the network of openings, the target being intended to be positioned on the side of the membrane which is opposite the microfluidic channel(s); one or more fluid feed means for feeding the or each microfluidic channel with fluid, at least one of these fluids comprising molecules for stimulating the target.

Microfluidic system for controlling the concentration of molecules for stimulating a target

Method of identifying a protocol at the origin of a data flow. The method of identifying a protocol giving rise to a packet flow comprises the following steps: &#x2014;a capture of the flow of the protocol to be identified, &#x2014;statistical classification of the flow, comprising an extraction of the classification parameters and a comparison of the classification parameters with statistical models constructed during a learning phase. The statistical classification comprises: &#x2014;a first phase of global statistical classification; and &#x2014;a step of synthesis of the results of the first and second classification phases so as to identify the protocol giving rise to the flow.

Method of identifying a protocol giving rise to a data flow

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Paris, France

Mathieu Morel