Illumina Corporation

Illumina Cambridge Limited

University of California

Mathieu Lessard-Viger

Tarun Kumar Khurana

Yir-Shyuan Wu

Jeffrey S Fisher

M Shane Bowen

Arnaud Rival

Lewis J Kraft

Xi-Jun Chen

Hui Han

Justin Fullerton

Yasaman Farshchi

Andrew Antony Brown

Wayne N George

Ludovic Vincent

Brian Douglas Mather

Adah Almutairi

Xavier Von Hatten

Gianluca Andrea Artioli

Clifford Lee Wang

Wangzhong Sheng

In an example, a target material is immobilized on two opposed sequencing surfaces of a flow cell using first and second fluids. The first fluid has a density less than a target material density and the second fluid has a density greater than the target material density; or the second fluid has a density less than the target material density and the first fluid has a density greater than the target material density. The first fluid (including the target material) is introduced into the flow cell, whereby at least some of the target material becomes immobilized by capture sites on one of the sequencing surfaces. The first fluid and non-immobilized target material are removed. The second fluid (including target material) is introduced into the flow cell, whereby at least some of the target material becomes immobilized by capture sites on another of the sequencing surfaces.

Immobilization in flow cells

A flow cell includes a support and a heteropolymer attached to the support. The heteropolymer includes an acrylamide monomer including an attachment group to react with a functional group attached to a primer, and a monomer including a stimuli-responsive functional group. The monomer including the stimuli-responsive functional group may be pH-responsive, temperature-responsive, saccharide-responsive, nucleophile-responsive, and/or salt-responsive.

Flow cell including a heteropolymer

An example of a flow cell includes a substrate, a plurality of chambers defined on or in the substrate, and a plurality of depressions defined in the substrate and within a perimeter of each of the plurality of chambers. The depressions are separated by interstitial regions. Primers are attached within each of the plurality of depressions, and a capture site is located within each of the plurality of chambers.

Flow cells

An example of a functionalized plasmonic nanostructure includes a plasmonic nanostructure core; a polymeric hydrogel attached to the plasmonic nanostructure core, the polymeric hydrogel having a thickness ranging from about 10 nm to about 200 nm; and a plurality of primers attached to side chains or arms of the polymeric hydrogel, wherein at least some of the plurality of primers are attached to the polymeric hydrogel at different distances from the plasmonic nanostructure core.

Functionalized plasmonic nanostructures

Flow cells using sequencing-ready nucleic acid fragments attached to carrier beads immobilized at capture sites of a plurality of chambers

Flow cells with chambers, depressions, and capture sites

Near infrared radiation at a wavelength that induces resonance in water is used to remotely activate thermal plasticization of polymeric particles to trigger the release of encapsulated molecules from the particles. Nanocarriers formed from biocompatible hydrophilic polymers may be used to deliver encapsulated molecules to tissue with a reversible transition that allows repeated activations for extended release of the payload.

Polymeric nanocarriers with light-triggered release mechanism

Growing community of inventors

San Diego, CA, United States of America

Mathieu Lessard-Viger