Roquette Freres

Mathieu Carpentier

Pierre Lanos

Fabrice Allain

Agnès Denys

Héla Hacine-Gherbi

Marc Biguet

Roselyne Bernard

Thierry Dupont

Sophie Duvet

The invention relates to a method for detecting contaminants of glucose polymers, said contaminants being capable of acting in synergy with one another so as to trigger an inflammatory reaction, characterized in that it comprises an in vitro inflammatory response test using modified cell lines.

Methods for detecting contaminants in solutions containing glucose polymers

The present invention relates to a biological method for the dosage of peptidoglycans in a sample, especially a sample of glucose polymers.

Biological dosage of peptidoglycans

The present invention relates to a biological method for assaying peptidoglycans (PGN) in a sample, particularly a sample of glucose polymers. The PGN assay includes: a) treating the glucose polymer sample by sonication, heating, and/or alkalizing; b) placing the treated sample or a dilution thereof in contact with a recombinant cell expressing an exogenous TLR2 (toll-like receptor 2) and a reporter gene directly dependent on the signaling pathway associated with the TLR2. The reporter gene codes for a colored or fluorescent protein or for a protein the activity of which is measurable with or without a substrate; c) measuring the reporter gene signal; and d) determining the amount of PGN in the sample using a standard curve of the correlation between the amount or PGN and the strength of the reporter gene signal.

Biological assay of peptidoglycans

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Saint Andre Lez Lille, France

Mathieu Carpentier