Pierre Fabre Medicament

Cemu Bioteknik Ab

Kabigen Ab

Dynal As

Other

Pharmacia & Upjohn Aktiebolag

Pharmacia Lkb Biotechnology Ab

Kabi Pharmacia Aktiebolag

Creative Peptides Sweden Ab

Cemu Bitecknik Ab

Cemv Bioteknik Ab

Mathias Uhlen

Stefan Stahl

Bjorn Nilsson

Hans Binz

Tomas Moks

Joakim Lundeberg

Lars B Abrahmsen

Thien Ngoc Nguyen

Thien Nguyen Ngoc

Christine Andreoni

Per-ANGke Nygren

Martin K Lindberg

Erik Holmgren

Erik Hornes

John Sjoquist

Bjorn Lowenadler

Per Jonasson

Per-Ake Nygren

Marianne Hansson

Per Ake Nygren

Stefan Stanghl

Ake Per Nygren

Sven Lofdahl

A complex of an immunogen and a support molecule, characterized in that the immunogen is coupled covalently to a support molecule, wherein the support molecule is a polypeptide fragment which is able to bind specifically to mammalian serum albumin is disclosed. The invention also relates to the use of such complexes to treat RSV infection, as well as vaccines derived from such complexes.

Complexes of immunogens derived from RSV surface glycoprotein G covalently coupled to a support molecule

The present invention provides a method of producing an insulin C-peptide, which comprises expressing in a host cell a multimeric polypeptide comprising multiple copies of the insulin C-peptide, and cleaving the expressed polypeptide to release single copies of the insulin C-peptide. Also provided are nucleic acid molecules, expression vectors and host cells, for use in such a method and the multimeric insulin C-peptide polypeptide expressed and cleaved in such a method.

Recombinant expression of insulin C-peptide

The invention provides a method of improving the binding of a series of consecutive nucleotide bases to a complementary target nucleic acid molecule in a sample, wherein said method comprises at least the step or steps of binding a complementary modular oligonucleotide of at least two parts (modules) including said nucleotide bases to adjacent stretches of said target nucleic acid molecule in said sample, especially methods of detection/isolation, particularly in the isolation of primer extension products and methods in which the modular oligonucleotide is a primer, modular oligonucleotides themselves and their use in methods of the invention.

Methods and kits for isolating primer extension products using modular oligonucleotides

Method for enhancing the immunogenicity of an immunogenic compound or

Method for obtaining a peptide derived from the respiratory syncytial

Tripartite recombinant DNA encoding fusion proteins which comprise three sequences, i.e., a signal peptide which is operable in a Gram positive bacterium, an immunogenic polypeptide linked thereto which is not normally expressed in a Gram positive bacterium, and a cell wall spanning and a membrane anchoring sequence, as well as their use in Gram positive bacteria to express the resultant fusion protein on their surface are described. The preferred cell wall spanning and anchoring polypeptides include Staphylococcus protein A and Streptococcus protein G.

Recombinant DNA coding for signal peptide, selective interacting

Bacterial receptor structures

The invention provides a method of determining the amount of target DNA in a sample which comprises the steps of (a) dividing the sample into a plurality of aliquots; (b) adding to each aliquot a known amount of competitor DNA which is the same as the target DNA except that it comprises a recognition site capable of being detected directly or indirectly by a labelled species whereby the ratio of target DNA to competitor DNA is different in the aliquots; (c) co-amplifying the target DNA and competitor DNA in each aliquot by the polymerase chain reaction (PCR) using at least one primer which permits immobilization of the co-amplified DNA, the annealing sites of any PCR primers used being outward of the recognition site; (d) labelling the amplified competitor DNA; (e) before or after step (d), immobilizing the co-amplified DNA where not already immobilized, via at least one PCR primer; (f) assessing the amount of label on the immobilized amplified competitor DNA in each aliquot to provide an indication of the amount of target DNA in the sample.

Competitive PCR for quantitation of DNA

A method of and kit for diagnosis of medical conditions by identification of specific DNA characterized in that DNA in a sample is subjected to initial amplification by the polymerase chain reaction (PCR) method using a first pair of primers specific to the target DNA and the amplified DNA so produced is further amplified by the PCR method using a second primer pair, one or both of which are different from either of said first primer pair and are specific to a sequence or sequences of the target DNA between the hybridization sites of said first primer pair, one of the primers of said second primer pair being immobilized on a solid support or being provided with means for subsequent attachment to a solid support and the other of said second pair of primers carrying a label or being provided with means for subsequent attachment to a label, said amplification being followed by separation of said solid support carrying said amplified DNA and detection of label attached thereto, one or both of said means for subsequent attachment comprising a distal DNA sequence carried by the said primer which does not hybridize with the target DNA but has a selective affinity for a binding partner attached to either a solid support or a label.

Solid phase diagnosis of medical conditions

Chemical method for the analysis of DNA sequences

Cloning method and kit

A process for preparing antibodies specific for an amino acid sequence which comprises immunizing a mammal with a fused protein comprising said amino acid sequence fused to an immunogenic IgG binding protein is disclosed.

Preparation of fused proteins, antibodies and processes therefore

The invention relates to a method of sequencing target DNA in which said DNA is provided in double stranded form immobilized on a solid support via one terminus of one of the two strands thereof and is then subjected to strand separation whereby the unattached strand is removed prior to sequencing the immobilized strand. The target DNA, e.g genomic DNA, may initially be amplified by the PCR method using at least one oligonucleotide primer which is either attached to a solid support or carries means for attachment to a solid support.

Method of sequencing DNA

Apparatus and method for automated purification of extra-chromo-somal

A process for expressing proteins in Gram(-) bacteria and providing for extracellular secretion thereof, comprising the steps: a) introducing into a Gram(-) bacterium a recombinant DNA construction comprising a promoter, a signal sequence enabling translocation and processing, and a structural gene encoding the desired protein to be expressed; b) cultivating the bacterium under conditions resulting in filamentous growth; and c) recovering the extracellularly secreted protein; a recombinant DNA construction comprising:

Method to export gene products to the growth medium of gram negative

Growing community of inventors

Upsala, Sweden

Mathias Uhlen