Carlsberg A/s

Carlsberg Breweries A/s

Massimiliano Carciofi

Ole Olsen

Søren Knudsen

Toni Wendt

Birgitte Skadhauge

Magnus Wohlfahrt Rasmussen

Hanne Cecille Thomsen

Finn Lok

Katarzyna Krucewicz

Lucia Marri

Alexander Striebeck

Hanne Thomsen

Christoph Dockter

Magnus Rasmussen

Pai Rosager Pedas

Rasmussen Magnus Wohlfahrt

Hanne Cecilie Thomsen

In traditional plant breeding approaches, chemical mutagenesis may be utilized to introduce nucleotide substitutions at random in the genome of a plant, i.e. without possibilities to control the sites of nucleotide changes. Because of genome complexities, the statistical probability is extremely little when it comes to finding a predetermined nucleotide substitution. The present invention, however, demonstrates how a novel, alternative use of digital polymerase chain reaction (dPCR), preferably droplet dPCR (ddPCR), is developed to exploit finding of specific nucleotide substitutions in mutated genes. The entire platform comprises a screening method with a library of mutagenized organisms, digital PCR-based systems and a set-up to propagate and analyze identified, mutated organisms.

Method to screen for a mutant within a population of organisms by applying a pooling and splitting approach

The invention relates to barley plants having a high α-amylase activity. The barley plants of the invention may for example carry a mutation in one or more α-amylase promoters, in the HIRT gene, in the HBL12 gene and/or in the WRKY38 gene. The invention further provides plant products prepared from said barley plants.

Barley with increased hydrolytic enzyme activity

Growing community of inventors

Copenhagen, Denmark

Massimiliano Carciofi