Danisco A/s

Dupont Nutrition Biosciences Aps

Masoud Rajabi Zargahi

Michael C Ward

Michael D Ward

Charlotte Horsmans Poulsen

Susan Mampusti Madrid

Cherry Lin

Mai Faurschou Isaksen

Preben Rasmussen

Rikke Høegh Lorentsen

Jacob B Visser

Jørn Borch Søe

Peter Plumstead

Jorn Borch Søe

Henrik Pedersen

Ronald Peter De Vries

Janne Brunstedt

Søren Kjaerulff

Luis Fernando Romero Millan

Rene Mikkelsen

Birgit Michelsen

J&oslash;rn Borch S&oslash;e

Rikke Hoegh Lorentsen

Jens Frisbaæk Sørensen

Jo Slashedrn So Slashede

Susan Mampusta Madrid

Tina Jørgensen

Claus Lindvald Johansen

Luis Fernando Romero Millán

Soren Kjaerulff

Jens Frisbæk SØRENSEN

Luis Fernando Romero MILLÁN

Susan Madrid

The present invention provides methods and uses for treating pyropheophytin-containing compositions, particularly in order to remove pyropheophytin therefrom. The compositions are typically plant, algal, or bacterial derived products such as vegetable oils. The method comprises a step of contacting the compositions with an enzyme having pyropheophytinase activity. Also provided are related apparatus and products for performing such methods and uses.

Method for treating pyropheophytin-containing compositions

The present invention provides a transformed or transfectedcell comprising one or more of: (i) at least one heterologous nucleotide sequence encoding a lipolytic enzyme comprising an amino acid sequence shown as SEQ ID NO: 1 or SEQ ID NO: 2, (ii) at least one heterologous nucleotide sequence encoding a lipolytic enzyme wherein the nucleotide sequence comprises the nucleotide sequence shown as SEQ ID NO: 3 or SEQ ID NO: 4, or (iii) at least one heterologous nucleotide sequence encoding a lipolytic enzyme wherein the nucleotide sequence comprises the nucleotide sequence which hybridizes to SEQ ID NO: 3 or SEQ ID NO: 4 or a nucleotide sequence which is at least 40% sequence identity to SEQ ID NO: 3 or SEQ ID NO: 4; and culturing the cell under conditions to allow for expression of the heterologous nucleotide sequence(s) encoding the lipolytic enzyme.

Method of producing a lipolytic enzyme

The present invention provides a transformed or transfectedcell comprising one or more of: (i) at least one heterologous nucleotide sequence encoding a lipolytic enzyme comprising an amino acid sequence shown as SEQ ID NO: 1 or SEQ ID NO:2, (ii) at least one heterologous nucleotide sequence encoding a lipolytic enzyme wherein the nucleotide sequence comprises the nucleotide sequence shown as SEQ ID NO: 3 or SEQ ID NO: 4, or (iii) at least one heterologous nucleotide sequence encoding a lipolytic enzyme wherein the nucleotide sequence comprises the nucleotide sequence which hybridizes to SEQ ID NO: 3 or SEQ ID NO: 4 or a nucleotide sequence which is at least 40% sequence identity to SEQ ID NO: 3 or SEQ ID NO: 4; and culturing the cell under conditions to allow for expression of the heterologous nucleotide sequence(s) encoding the lipolytic enzyme.

The present invention relates to a feed supplement comprising a phytase and a lipolytic enzyme, wherein said lipolytic enzyme has lipase activity at a pH in the range of about pH1.5 to about pH3.5.

Feed supplement with lipase and phytase

Feed supplement

A method is described for releasing a soluble or membrane associated intracellular protein of interest (POI) comprising the steps of: providing a cell comprising a soluble or membrane associated intracellular POI; contacting the cell with a membrane extracting composition; and causing the POI to be released from the cell under conditions sufficient for the specific release of the POI and in a soluble form.

Method of extracting recombinant hexose oxidase

Method of improving the rheological properties of a flour dough and the quality of bread, alimentary paste products, noodles and cakes wherein glycerol oxidase or a combination of glycerol oxidase and a lipase is added to the dough and dough improving compositions comprising these enzymes. The strength of (B/C ratio) and the gluten index of the dough was improved and in the resulting products the improvements were higher specific volume, increased crumb pore homogeneity and reduced average crumb pore diameter.

Method for preparing flour doughs and products made from such doughs using lipase

The present invention relates to the isolation of and characterization of a novel lipase inhibitor and its effect on different lipases. The present invention also relates to the use of a lipase inhibitor as a screen for lipases. The present invention also relates to the use of the inhibitor and/or lipases identified by a lipase inhibitor in food and/or feed technologies.

Protein lipase inhibitor for flour and dough

Recombinantly produced &agr;-glucuronidases which are useful in food manufacturing and as a feed additive to enhance the utilization of the feed components, and in other industrial applications such as pulp processing are provided. Genes coding for such enzymes can e.g. be isolated from Aspergillus sp. including or

&agr;-glucuronidases of aspergillus, production thereof and their uses

A method of improving the rheological properties of a flour dough and the quality of bread, alimentary paste products, noodles and cakes produced therefrom, wherein a combination of (a) a glycerol oxidase which does not require a co-factor to oxidize glycerol, and (b) a lipase, is added to the dough to produce a synergistic effect upon said rheological properties; and dough improving compositions containing such components.

Method for preparing flour doughs and products made from such doughs using glycerol oxidase and lipase

An enzyme system is described that is useful for preparing food and feed. One enzyme of that system is obtainable from Aspergillus. That enzyme has the following characteristics: a MW of from 29 kDa to 36 kDa as measured on a SDS-Phastgel (10-15%) or about 30 kDa; a pI value of about 3-4; ferulic acid esterase activity; a pH optimum of about 5; and a temperature optimum of from about 50 to about 60.degree. C.

Enzyme system comprising ferulic acid esterase from Aspergillus

Growing community of inventors

Aarhus N, Denmark

Masoud Rajabi Zargahi