Kyoto Daiichi Kagaku Co., Ltd.

Arkray, Inc.

The Doshisha

Masayuki Yagi

Nobuo Kato

Yoshiki Tani

Yasuyoshi Sakai

Harumi Uenoyama

Yoshinori Yamaguchi

Kaori Ishimaru

Fumiyo Funatsu

Satoshi Yonehara

Xiaoming Dou

Dou Xiaoming

Emi Ashibe

Hiroshi Fukuya

Toshikatsu Sakai

Yung Xiang Wang

Satoshi Cyousa

Yoshikazu Yonei

Hiroshige Kawai

Masako Shoshihara

Yuko Saito

Satoko Togashi

The present invention relates to a novel enzyme (&agr;-GARE) which releases an amino acid residue having a glycated &agr;-amino group (&agr;-GA) from a glycated protein etc. and to bacterial strains producing the same. Examples of the bacterial strains include KDK1004 (FERM BP-7041). The &agr;-GARE is contained in the culture supernatant of this strain and &agr;-GA can be released from a glycated peptide by using the same, as shown in FIG.

&agr;-glycated amino acid releasing enzyme

The present invention relates to a novel enzyme (&agr;-GARE) which releases an amino acid residue having a glycated &agr;-amino group (&agr;-GA) from a glycated protein etc. and to bacterial strains producing the same. Examples of the bacterial strains include KDK1002 (FERM P-17135) and KDK1001 (FERM P-17133). The &agr;-GARE is contained in the culture supernatant of these strains and &agr;-GA can be released from a glycated peptide by using the same, as shown in FIG.

Enzyme

Fructosyl amino acid oxidase, process for producing the same, and method

A novel fructosyl amino acid oxidase derived from genus Fusarium or Gibberella, a process for producing the enzyme, an assay of an amadori compound using the enzyme, a reagent or a kit containing the enzyme, a process for producing fructosyl lysine and/or fructosyl N.sup..alpha. -Z-lysine as a substrate of a fructosyl amino acid oxidase, which is useful for screening and/or culturing a microorganism capable of producing the enzyme, and a medium containing fructosyl lysine and/or fructosyl N.sup..alpha. -Z-lysine for screening and/or culturing a microorganism capable of producing a fructosyl amino acid oxidase is provided.

Fructosyl amino acid oxidase and process for producing the same

A sample solution is stored in a total reflection cell, and a measuring beam is introduced from an incident optical system to be totally reflected, for measuring a total reflection absorption spectrum. Hydrogen peroxide contained in the sample solution is determined on the basis of absorbance at the position of any absorption peak of the spectrum which is present at 1200 to 1500 cm.sup.-1 or 2600 to 3000 cm.sup.-1. Hydrogen peroxide contained in an aqueous solution can be simply quantitatively analyzed through optical analysis.

Method of and apparatus for determining hydrogen peroxide

A novel fructosyl amino acid oxidase derived from genus Penicillium, which is active on both of fructosyl lysine and fructosyl valine, a process for producing the enzyme, an assay of an amadori compound using the enzyme, a reagent or a kit containing the enzyme is provided.

Acid is added to a target sample such as urine, blood plasma or serum for converting mevalonic acid contained in the sample to lactone mevalonate, and the sample is irradiated with near infrared Raman excitation light, for determining mevalonic acid by measuring generated Raman scattered light. This method employs no mediatory reaction with simple pretreatment, requires no high-priced measuring apparatus, and can determine mevalonic acid in a short time.

Optical measuring method for mevalonic acid

An excitation beam generated from a light source is separated into a measuring beam and a reference beam by an optical path adjusting optical system including a beam splitter, so that the measuring beam is applied to a sample in a cell. Raman scattering light which is generated from the sample is detected by a spectral detector including a spectroscope through a scattering light path adjusting optical system and a wavelength selector. In a spectrum obtained by the spectral detector, a peak of Raman scattering in which wavenumber shift from the wavelength of the excitation beam is present at 800 to 920 cm.sup.-1 is employed to make quantitative measurement of hydrogen peroxide. Hydrogen peroxide contained in an aqueous solution can be simply quantitatively analyzed through optical analysis means.

Method of and apparatus for determining hydrogen peroxide by raman

In order to quantitatively measure an Amadori compound by a simple optical method utilizing light scattering, a sample containing an Amadori compound which is stored in a cell is irradiated with excitation light from an He--Ne laser unit so that scattered light from the sample is received and separated into its spectral components for obtaining a light scattering spectrum, and a light scattering peak existing at 820 to 840 cm.sup.-1, 1655 to 1660 cm.sup.-1, 2000 to 2020 cm.sup.-1, 2080 to 2100 cm.sup.-1, 2460 to 2470 cm.sup.-1 or 2530 to 2600 cm.sup.-1 in shift wavenumber with respect to the excitation wavelength in the light scattering spectrum is detected by a detector. The saccharide concentration or the saccharification ratio of the Amadori compound is measured by a calibration curve through the peak intensity or the peak integral value of the light scattering peak.

Method of measuring Amadori compound by light scattering

Growing community of inventors

Kyoto, Japan

Masayuki Yagi