Chugai Seiyaku Kabushiki Kaisha

Sony Corporation

Kagoshima University

Tokyo Metropolitan Institute of Medical Science

Miyazaki Prefectural Industrial Support Foundation

Masayuki Sudo

Yasunobu Amano

Hiroshi Sakamoto

Michinori Kohara

Akira Nakayama

Hirohito Tsubouchi

Tomohisa Uba

Kazuo Omae

Hirofumi Uto

Takeshi Okanoue

Yo-ichi Ishida

Yoshiro Ishikawa

Yuko Sato

Ichiro Iwaki

The present inventors focused on siE sequences that have been thought to show RNAi activity against HCV viral RNAs, and mainly selected the D5-50 and D5-197 regions present within the IRES region, and carried on the analysis. As a result, the present inventors successfully identified siRNA sequences that exhibit a more effective RNAi activity against hepatitis C virus RNAs. Furthermore, the siRNAs were demonstrated to have a significant inhibitory effect on HCV propagation in an in vivo system.

Oligoribonucleotide or peptide nucleic acid capable of inhibiting activity of hepatitis C virus

Disclosed are: a marker for the diagnosis of a liver disease, which can determine the disease in a simple manner; an antibody directed against the marker; a diagnostic agent; a diagnosis method; and a method for marker detection in blood or serum. Proteome analysis revealed that quantities of the full-length kininogen and three partial peptides thereof (sequence A: position-440 to position-456, sequence B: position-439 to position-456, and sequence C: position-438 to position-456) in sera of patients with non-alcoholic fatty liver disease are significantly different from those in sera of healthy individuals; and a diagnostic agent and a detecting method for the non-alcoholic fatty liver disease that can be conveniently used for medical examination are established. The use of a combination of a kininogen-based marker and a C4-based marker (the full length sequence or partial peptides thereof) enables identification of chronic hepatitis and an asymptomatic virus carrier, as well as non-alcoholic fatty liver disease.

Biomarker for diagnosis of liver disease

The present inventors examined the inhibitory activity against HCV replicon of myriocin, fumonisin B1, and ceramide trafficking inhibitor HPA-12, which are compounds derived from microorganisms such as those of the genus, and found that these compounds have the effect of inhibiting the replication of HCV replicon RNAs or inhibiting the expression of HCV proteins. The present inventors further performed serine palmitoyltransferase knockdown experiments using siRNAs, and results showed that HCV replicon activity and HCV protein expression were significantly inhibited in cells with suppressed LCB1 expression. Thus, sphingolipid biosynthesis was found to be involved in HCV infection. This showed that HCV infection can be treated or prevented by inhibiting enzyme activities involved in the process of sphingolipid biosynthesis by adding compounds or knocking down a gene.

Pharmaceutical agents for treating HCV infections

A cathode-ray tube in which outer convergence plates as constituents of the convergence plate assembly in the electron gun are supplied with individual voltages so that the horizontal misconvergence at the center portion and at the peripheral portions of the screen are positively corrected and in the arrangement the anode button is provided with a plurality of voltage supply pins. Further, the end portions of the two lead wires which are introduced form the anode button are opened outwardly so that the lead wires are prevented from coming in contact with the bead glass.

Cathode-ray tube with internal insulated electrical conductors

A cathode-ray tube which has an electron gun and a correcting unit with a magnetic device electrically coupled to a final electrode of the electron gun so as to selectively apply a uniform magnetic field to required ones of a plurality of electron beams, so as to thereby correct the path of a selected electron beam without causing distortion in the shape of the beam spot.

Cathode-ray tube with electrostatic convergence means and magnetic

Growing community of inventors

Kanagawa, Japan

Masayuki Sudo