National Institute of Advanced Industrial Science and Technology

Kumiai Chemical Industry Co., Ltd.

National Institute of Technology and Evaluation

Honda Motor Co., Ltd.

National Research Institute of Brewing

Kaneka Corporation

Tohoku University

Noda Institute for Scientific Research

Kikkoman Corporation

Amano Enzyme Inc.

Masayuki Machida

Kiyoshi Asai

Keietsu Abe

Motoaki Sano

Kiyoshi Kawai

Naotake Ogasawara

Katsuya Gomi

Hideki Nagasaki

Taishin Kin

Akira Hosoyama

Osamu Akita

Takashi Kubo

Tomonori Fujioka

Shigenari Yamaguchi

Shigenobu Mitsuzawa

Satoru Shinkawa

Maiko Tanaka

Satoru Kuhara

Mahoko Kuninaga

Hideaki Koike

Hiroko Hagiwara

Yasuji Koyama

Teruyuki Nishi

Kenichiro Matsushima

Akira Yoshimi

Maiko Umemura

Shotaro Yamaguchi

Tsutomu Masuda

Katsuhiko Kitamoto

Kotaro Ito

Atsuki Toumoto

Genryou Umitsuki

Osamu Hatamoto

Kensuke Yuuki

Manabu Arioka

Satoru Hisahara

Misao Sunagawa

Tasuku Nakajima

Hiroshi Maeda

Tomomi Uchikawa

Touru Ito

Hitoshi Tainaka

Chitoshi Oka

Akimitsu Tanaka

Genryou Umitsüki

Masahiro Ogawa

Tomomi Ichikawa

This invention is intended to identify a gene cluster involved in biosynthesis of a cyclic peptide compound produced by a filamentous fungus of thespecies and to establish a system for synthesizing such cyclic peptide compound. The gene is composed of a first module to a tenth module and encodes a protein having activity of synthesizing a nonribosomal peptide constituting a basic peptide backbone of a cyclic peptide compound produced by a filamentous fungus of thespecies.

Gene involved in synthesis of cyclic peptide compound, method for producing cyclic peptide compound using the same, and transformant comprising the same

An object of the present invention is to identify an enzyme having activity of synthesizing dihydrotentoxin that is a tentoxin precursor and an enzyme having activity of synthesizing tentoxin using dihydrotentoxin as a substrate. The present invention concerns a tentoxin synthesis-related gene encoding a protein comprising the amino acid sequence of SEQ ID NO: 16 and having activity of nonribosomal peptide synthesis of dihydrotentoxin and a tentoxin synthesis-related gene encoding a protein comprising the amino acid sequence of SEQ ID NO: 18 and having activity of converting dihydrotentoxin to tentoxin.

Method for producing dihydrotentoxin

Tentoxin synthesis gene, a method for producing tentoxin or dihydrotentoxin using the same, and a transformant comprising the same

To provide a base sequence for protein expression that can increase the yield of protein such as diastatic enzyme by further activating a promoter of a particular gene. A base sequencefor protein expression includes: a geneencoding protein; a promoterof the gene, the promoter being linked upstream of the gene; and a cis elementwhose activity is improved by an artificial transcription factor, the cis element being linked further upstream of the promoter. The cis elementis represented by SEQ ID NO: 1.

Base sequence for protein expression and method for producing protein using same

This invention provides a novel means that allows efficient secretion and production of a target protein in a host cell. This invention concerns a novel polypeptide having an activity of improving the secretion productivity of a target protein, a gene comprising a nucleotide sequence encoding such polypeptide, a vector comprising such gene, a transformant obtained by transforming a host cell with such vector, and a method for producing a protein comprising a step of culturing such transformant and recovering a target protein from the culture product.

Polypeptide, and use thereof

Nucleic acid sequence for protein expression

Disclosed are: transcription regulatory factors capable of regulating the transcription or expression of genes for mannanases or cellulases, as mentioned below; and others. Specifically disclosed is a protein selected from the following proteins (a), (b) and (c): (a) a protein comprising the amino acid sequence depicted in SEQ ID NO:2; (b) a protein which comprises an amino acid sequence produced by deleting, substituting or adding one or several amino acid residues (e.g., 1 to 5 amino acid residues) in the amino acid sequence depicted in SEQ ID NO:2 and which is capable of regulating the transcription of genes for mannanases or cellulases; and (c) a protein which comprises an amino acid sequence having a 70% or higher sequence identity to the amino acid sequence depicted in SEQ ID NO:2 and which is capable of regulating the transcription of genes for mannanases or cellulases, or a partial fragment of the protein. Also specifically disclosed are a gene encoding the protein, and others.

Transcription regulatory factors for mannanases or cellulases, and genes for the transcription regulatory factor

To improve the activity of a Koji mold protease in a solid or liquid culture medium in the production of foods (e.g., a seasoning), pharmaceuticals (e.g., a digestive agent), protease for use in a detergent and the like. Disclosed are a recombinant vector having capability of increasing the secretion of the Koji mold protease, a Koji mold which is transformed with the vector and has an increased expression of a gene for a protease or an increase secretion of the same, a method for the production of a protease by using the transformed Koji mold, and the like.

Recombinant vector capable of increasing secretion of Koji mold protease

It is intended to provide koji mold-origin phospholipase Aand a DNA encoding it. Namely, phospholipase Acomprising the following protein (a) or (b): (a) a protein having an amino acid sequence represented by SEQ ID NO: 1 or 2; and (b) a protein having an amino acid sequence derived from an amino acid sequence represented by SEQ ID NO: 1 or 2 by a partial modification and serving as phospholipase A.

[object Object]

Lysyl oxidase derived from filamentous fungi and a DNA encoding thereof are provided. Lysyl oxidase including a protein described in of the following (a) or (b): (a) a protein having an amino acid sequence set forth in SEQ ID NO: 2; or (b) a protein having an amino acid sequence obtained by modifying a part of the amino acid sequence set forth in SEQ ID NO: 2, and functioning as lysyl oxidase.

Fungus-origin lysyl oxidases

The purpose of the present invention is to isolate a gene encoding novel exo-, or endo-&#x3b2;--glucanase, to obtain a microorganism having an enhanced expression of said gene and to degrade &#x3b2;--glucan to its low molecular weight form by means of said enzymes. The present invention relates to a gene or DNA encoding novel enzymes having &#x3b2;--glucanase activity (exo-, or endo-&#x3b2;--glucanase), a recombinant expression vector comprising them, a microorganism having the recombinant expression vector, the novel enzymes having &#x3b2;--glucanase activity, and a method for the production of said enzymes.

Genes of cell wall-degrading enzymes derived from aspergillus, and method for the production the enzymes

A completely novel glutaminase is provided: (a) a protein consisting of an amino acid sequence represented by SEQ ID NO: 2, or (b) a protein consisting of an amino acid sequence derived from the amino acid sequence represented by SEQ ID NO: 2 by deletion, substitution or addition of one or more amino acids, and possessing glutaminase activity. This protein is a novel glutaminase possessing excellent thermostability and the like.

Thermostable glutaminase and thermostable glutaminase gene

Growing community of inventors

Sapporo, Japan

Masayuki Machida