Eisai Company, Limited

Masayuki Konishi

Takashi Sato

Hiroshi Yoshino

Yutaka Tsuchiya

Masahiko Tsujii

Yoshihiko Hisatake

Atsushi Koiwa

Mikio Uchiyama

Akira Hashimoto

Koichi Ogawa

Tsunehiko Kataoka

Yoshiaki Nagara

Purified secretin is obtained by subjecting crude secretin to reversed-phase high-performance liquid chromatography using, as an eluant, a solvent mixture of an acid or salt thereof and an organic solvent.

Process for purifying secretin

A process is disclosed for purifying elastase by precipitating elastase from an aqueous, elastase-containing solution, in which the electroconductivity of said aqueous solution is adjusted to 3 millimhos per centimeter or less and the pH of the solution is adjusted to a value of from 2 to 5. The electroconductivity of said aqueous solution is preferably adjusted to 3 millimhos per centimeter or less by means of ultrafiltration.

Process for purifying elastase

A heptacosapeptide having the following structural formula:

Heptacosapeptide

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Ichinomiya, Japan

Masayuki Konishi