Research Institute of Innovative Technology for the Earth

Green Phenol Development Co., Ltd.

Sumitomo Bakelite Company Limited

Green Chemicals Co., Ltd.

Sumitomo Rubber Industries Limited

Sharp Corporation

Sharp Kabushiki Kaisha Corporation

Research Institute of Innovation Technology for the Earth

Masayuki Inui

Hideaki Yukawa

Masako Suda

Kazumi Hiraga

Akihito Yoshida

Takahisa Kogure

Ryoma Hashimoto

Naoto Torata

Takeshi Kubota

Junya Ishida

Keitarou Fujikura

Toru Jojima

Masayoshi Hashizume

Shikiko Murakami

Satoshi Hasegawa

Kaori Nakata

Shohei Okino

Yuko Ikenaga

Naoto Kato

Provided is a microorganism that is able to produce 2-phenylethanol at a high concentration, and a method of efficiently producing 2-phenylethanol by using a saccharide as a raw material. Provided is a coryneform bacterium transformant in which a shikimate pathway is activated, and further, a gene that encodes an enzyme having phenylpyruvate decarboxylase activity is introduced in such a manner that the gene can be expressed. Also provided is a 2-phenylethanol producing method that includes causing the coryneform bacterium transformant according to the present disclosure to react in water containing a saccharide.

Coryneform bacterium transformant and method for producing 2-phenylethanol using same

Provided is a method for improving productivity in producing an organic compound in a bacterium that originally does not have an inherent ED pathway. In one aspect, provided is a transformant of a coryneform bacterium that is obtained by introducing the Entner-Doudoroff pathway into the coryneform bacterium as a host. In another aspect, provided is a transformant of a coryneform bacterium that is obtained by introducing, into a coryneform bacterium as a host a gene in which an enzyme having glucose-6-phosphate dehydrogenase activity is encoded, a gene in which an enzyme having 6-phosphogluconate dehydratase activity is encoded, and a gene in which an enzyme having 2-keto-3-deoxy-6-phosphogluconate aldolase activity is encoded.

Transformant having Entner-Doudoroff pathway and production method for organic compound using same

Provided is a transformant of a microorganism that has improved catechol productivity.

Transformant of coryneform bacterium and production method for useful compound using same

Provided is a microorganism that is capable of efficiently producing para-aminobenzoic acid (4-ABA) or a salt thereof, using saccharides as raw materials, and a method for efficiently producing 4-ABA or a salt thereof by using this microorganism. A transformant obtained by introducing, into a coryneform bacterium, a gene that encodes 4-amino-4-deoxychorismate lyase, a gene that encodes a para-aminobenzoate synthase component I, and a gene that encodes a para-aminobenzoate synthase component II, is capable of efficiently producing 4-ABA or a salt thereof from saccharides.

Coryneform bacterial transformant and method for producing 4-aminobenzoic acid or salt thereof using same

Provided is a microorganism that is able to efficiently produce protocatechuic acid or a salt thereof by using a saccharide as a raw material, and a method of efficiently producing protocatechuic acid or a salt thereof by using the microorganism. Provided is a transformant having protocatechuic acid producing ability, subjected to modifications of enhancement of 3-dehydroshikimate dehydratase activity; enhancement of chorismate pyruvate lyase activity; and enhancement of 4-hydroxybenzoate hydroxylase activity. Also provided is a method of producing protocatechuic acid or a salt thereof, including the step of culturing the transformant in a reaction solution containing a saccharide so as to cause the transformant to produce protocatechuic acid or a salt thereof.

Transformant, and method for producing protocatechuic acid or salt thereof using same

The mutant chorismate-pyruvate lyase (A) or (B) as described below is capable of producing 4-hydroxybenzoic acid or a salt thereof with sufficient practical efficiency.

Transformant for producing 4-hydroxybenzoic acid or salt thereof

The mutant chorismate-pyruvate lyase (A) or (B) as described below is capable of producing 4-hydroxybenzoic acid or a salt thereof with sufficient practical efficiency. (A) A mutant chorismate-pyruvate lyase obtained by replacing the valine at position 80 in a chorismate-pyruvate lyase (ubiC) fromconsisting of the amino acid sequence of SEQ ID NO: 1 with one or more other amino acids. (B) A mutant chorismate-pyruvate lyase obtained by replacing an amino acid in another chorismate-pyruvate lyase, the amino acid being at a position enzymologically homologous with that of the above valine, with one or more other amino acids.

Highly active mutant enzyme for producing 4-hydroxybenzoic acid or salt thereof

A coryneform bacterium transformant engineered by the following (A) to (D): (A) enhancement of 3-deoxy-D-arabino-heptulosonate-7-phosphate (DAHP) synthase activity; (B) prevention, inhibition, or reduction of intracellular sugar uptake mediated by phosphotransferase system (PTS); (C) enhancement of intracellular sugar uptake activity mediated by a sugar transporter different from phosphotransferase system and enhancement of glucokinase activity; and (D) enhancement of glyceraldehyde-3-phosphate dehydrogenase (GAPDH) activity is capable of efficiently producing shikimic acid or the like from a sugar.

Coryneform bacterium transformant and process for producing organic compound using the same

A transformant constructed by introducing a gene which encodes an enzyme having chorismate-pyruvate lyase activity into a coryneform bacterium as a host is capable of efficiently producing 4-hydroxybenzoic acid or a salt thereof from a sugar. When the transformant is cultured under aerobic conditions where the transformant does not grow, 4-hydroxybenzoic acid or a salt thereof can be produced in a particularly efficient manner.

Coryneform bacterium transformant and process for producing 4-hydroxybenzoic acid or salt thereof using the same

An objective of the present invention is to provide a microorganism capable of efficiently producing aniline from aminobenzoic acid, and a process for efficiently producing aniline from aminobenzoic acid. To achieve the objective, provided is an aniline-producing transformant constructed by introducing a gene which encodes an enzyme having aminobenzoate decarboxylase activity into a coryneform bacterium as a host, characterized in that the enzyme having aminobenzoate decarboxylase activity is composed of an amino acid sequence which is the same as that represented by SEQ ID NO: 2 except for having a mutation of at least proline (P) at position 309 from the N terminus.

Coryneform bacterium transformant with improved aniline productivity and process for producing aniline using the same

Provided is an aniline-producing transformant constructed by introducing a gene which encodes an enzyme having aminobenzoate decarboxylase activity into a coryneform bacterium as a host. Also provided is a process for producing aniline, which comprises a step of allowing the transformant to react in a reaction mixture containing aminobenzoic acid, an ester thereof, and/or a salt thereof under reducing conditions, and a step of recovering aniline from the reaction mixture.

Coryneform bacterium transformant and process for producing aniline using the same

Provided is a phenol-producing transformant constructed by transferring a gene which encodes an enzyme having chorismate-pyruvate lyase activity and a gene which encodes an enzyme having 4-hydroxybenzoate decarboxylase activity into a coryneform bacterium as a host. Also provided is a process for producing phenol, which comprises a step of allowing the transformant to react in a reaction mixture containing a saccharide under reducing conditions, and a step of collecting phenol from the reaction mixture.

Coryneform bacterium transformant and process for producing phenol using the same

A phenol-producing transformant is provided which can efficiently produce phenol from a saccharide. A gene which encodes an enzyme having tyrosine phenol-lyase activity is transferred intoas a host. A process for producing phenol is provided having a step of reacting the transformant in a reaction mixture containing a saccharide under reducing conditions, and a step of collecting phenol from the reaction mixture.

A transformant obtainable by introducing one or more of the following DNAs (a), (b), and (c) into a coryneform bacterium as a host. (a) A DNA which encodes acetohydroxy acid synthase derived fromand which has a mutation changing the glycine at position 156 to glutamic acid (G156E) in an amino acid sequence encoded by the DNA, or an analog thereof. (b) A DNA which encodes acetohydroxy acid isomeroreductase derived fromand which has mutations changing the serine at position 34 to glycine (S34G), the leucine at position 48 to glutamic acid (L48E), and the arginine at position 49 to phenylalanine (R49F) in an amino acid sequence encoded by the DNA, or an analog thereof.(c) A DNA which encodes leucine dehydrogenase derived from, or an analog thereof.

Coryneform bacterium transformant and process for producing valine using the same

Provided is a phenol-producing transformant constructed by transferring a gene which encodes an enzyme having 4-hydroxybenzoate decarboxylase activity intoas a host. Also provided is a process for producing phenol, which comprises a step of allowing the transformant to react in a reaction mixture containing 4-hydroxybenzoate or a salt thereof under reducing conditions, and a step of collecting phenol from the reaction mixture.

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Kyoto, Japan

Masayuki Inui