Ajinomoto Co., Ltd.

Masayuki Araki

Yusuke Takahashi

Tsuyoshi Nakamatsu

Takahiro Asano

Masakazu Sugimoto

Akihiro Watanabe

Yasuhiko Yoshihara

Shintaro Iwatani

Wataru Hibino

Satoshi Okutani

Kazuya Kondo

Fumito Ohnishi

A method is described for producing an L-amino acid or a nucleic acid by culturing a microorganism having an ability to produce the L-amino acid or nucleic acid in a liquid medium in a fermentation tank containing a stirring impeller, and optionally adding seed crystals to the medium as required to produce and accumulate crystals of the L-amino acid or nucleic acid in the medium, and collecting crystals of the L-amino acid or nucleic acid from the culture. The power density of the stirring impeller is controlled to be 2.4 kW/mor lower after either precipitation of the crystals or addition of the seed crystals.

Method for producing an L-amino acid or a nucleic acid

A coryneform bacterium harboring an aspartokinase in which feedback inhibition by L-lysine and L-threonine is substantially desensitized, and comprising an enhanced DNA sequence coding for a dihydrodipicolinate reductase, an enhanced DNA sequence coding for dihydropicolinate reductase, an enhance DNA sequence coding for dihydropicolinate synthase, an enhanced DNA sequence coding for diaminopimelate decarboxylase and an enhanced DNA sequence coding for aspartate aminotransferase; a method for producing L-lysine comprising the steps of cultivating the coryneform bacterium in an appropriate medium to allow L-lysine to be produced and accumulated in a culture of the bacterium, and collecting L-lysine from the culture; and a recombinant DNA usable for production of the coryneform bacterium.

Method for producing L-lysine

Growing community of inventors

Kawasaki, Japan

Masayuki Araki