Toyota Jidosha Kabushiki Kaisha

Kyoto University

Masayoshi Muramatsu

Masakazu Ito

Shusei Obata

Chikara Ohto

Satoshi Yoneda

Jun Ogawa

Satoru Nishimura

Hiroyuki Enoki

Shigenobu Kishino

Hiroaki Koishihara

Masanori Honjo

Susumu Yui

Sakayu Shimizu

Tanetoshi Koyama

Ayumi Koike

Kyozo Ogura

Hiromi Kambe

Yenwin Cho

Naoto Shimizu

Tomohisa Kuzuyama

Kazuhiko Totsuka

Katsunori Kohda

Takashi Shimamura

Kiyohiko Nishi

Kazuyo Suzuki

Masahiro Sugimura

Ai Inoue

This invention intends to develop many DNA markers for a plant of the genusand detect powdery mildew resistance with high precision by using the many DNA markers. The marker associated with powdery mildew resistance in a plant of the genuscomprises a continuous nucleic acid region sandwiched between the nucleotide sequence as shown in SEQ ID NO: 1 and the nucleotide sequence as shown in SEQ ID NO: 19 in the chromosome of the plant of the genus

Marker associated with powdery mildew resistance in plant of genusand use thereof

The present disclosure provides a novel aldehyde synthase gene having activity of synthesizing a medium-chain aldehyde. Such gene encodes a protein comprising the amino acid sequence as shown in SEQ ID NO: 2.

Aldehyde synthase gene, recombinant microorganism comprising the same, and method for producing alkane using the same

The present disclosure is intended to identify a substitution mutation that improves enzyme activity of a decarbonylase. An amino acid corresponding to an amino acid 90, 107, 163, or 171 in SEQ ID NO: 2 is to be substituted with an amino acid exhibiting a higher degree of hydrophobicity.

Mutant decarbonylase gene, recombinant microorganism comprising the mutant decarbonylase gene, and method for producing alkane

This invention intends to develop many DNA markers for a plant of the genusand identify everbearing lines with high precision by using the many DNA markers. The marker associated with everbearing properties in a plant of the genuscomprises a continuous nucleic acid region sandwiched between the nucleotide sequence as shown in SEQ ID NO: 1 and the nucleotide sequence as shown in SEQ ID NO: 5 in the chromosome of the plant of the genus

Marker associated with everbearing properties in plant of genusand use thereof

This invention provides a recombinant microorganism into which an acyl-CoA reductase exhibiting excellent activity in a reduction reaction using acyl-CoA as a substrate has been introduced. Such recombinant microorganism comprises a nucleic acid encoding an acyl-CoA reductase comprising any of characteristic Common sequences 1 to 3 introduced into a host microorganism.

Recombinant microorganism and method for producing a substance using the same

A hydrocarbon synthase gene encoding protein having excellent capacity to synthesize a hydrocarbon such as alkane and novel functions is provided. The gene encodes a protein comprising an amino acid sequence comprising a motif sequence shown in SEQ ID NO: 1 and having activity of synthesizing a hydrocarbon with a carbon number one less than that of an aldehyde compound from the aldehyde compound.

Hydrocarbon synthase gene and use thereof

A method for producing a linear hydrocarbon, including reacting a linear aliphatic aldehyde in the presence of at least one metal ion selected from the group consisting of a vanadium ion, a manganese ion, an iron ion, a cobalt ion, an iridium ion, a copper ion, and a thallium ion.

Method for producing hydrocarbon

This invention provides a recombinant microorganism into which an acyl-CoA reductase exerting excellent activity in a reduction reaction involving the use of acyl-CoA as a substrate has been introduced. Such recombinant microorganism comprises a nucleic acid encoding a protein (a) or (b) below introduced into a host microorganism: (a) a protein comprising the amino acid sequence of SEQ ID NO: 2; or (b) a protein comprising an amino acid sequence having 70% or higher identity to the amino acid sequence of SEQ ID NO: 2 and having activity for synthesizing an aldehyde compound from acyl-CoA.

Substance productivity is improved by introducing a metabolic pathway for synthesis of acetyl-CoA or acetic acid from glucose-6-phosphate into yeast. Acetic acid productivity, acetyl-CoA productivity, and productivity of a substance made from acetyl-CoA-derived are improved by attenuating genes involved in the glycolytic system of yeast and introducing a phosphoketolase gene into the yeast.

Recombinant yeast and substance production method using the same

Isopropanol is produced with good productivity via fermentation processes. Specifically, isopropanol is produced at a high level in a medium by culturing recombinant yeast into which an acetoacetyl CoA synthase gene and a group of genes (isopropanol synthesis-related gene group) encoding a group of enzymes for synthesis of isopropanol from acetoacetyl CoA have been introduced.

Method for producing isopropanol and recombinant yeast capable of producing isopropanol

This invention relates to a recombinant microorganism having improved butanol production capacity and butanol production with the use of such recombinant microorganism with good efficiency. In this invention, the acetoacetyl-CoA synthase gene encoding an enzyme capable of synthesizing acetoacetyl-CoA from malonyl-CoA and acetyl-CoA and a group of genes involved in butanol biosynthesis that enables synthesis of butanol from acetoacetyl-CoA are introduced into a host microorganism.

Recombinant microorganism having butanol production capacity and butanol production method

Aliphatic polyester productivity is improved for production of aliphatic polyester using a recombinant microorganism. A recombinant microorganism prepared by introducing a gene encoding a protein having activity of converting lactic acid to lactic-acid CoA and a gene encoding a protein having activity of synthesizing polyhydroxyalkanoate using hydroxyacyl CoA as a substrate into a host microorganism is cultured and then aliphatic polyester is recovered from the medium.

Growing community of inventors

Miyoshi, Japan

Masayoshi Muramatsu