Riken Corporation

Sumitomo Pharma Co., Ltd.

Sumitomo Chemical Company, Limited

Sumitomo Dainippon Pharma Co., Ltd.

Nidek Co., Ltd.

Kyoto University

Healios K.k.

Racthera Co., Ltd.

Osaka University

Masayo Takahashi

Suguru Yamasaki

Yoshiki Sasai

Atsushi Kuwahara

Yasushi Hiramine

Satoshi Okamoto

Hiroyuki Kamao

Michiko Mandai

Noriko Sakai

Toshifumi Sumiya

Tsuguo Nanjo

Jun Takahashi

Mototsugu Eiraku

Masanori Sawada

Daisuke Doi

Hanako Ikeda

Osamu Mita

Koji Osawa

Kenji Yoshida

Akishi Onishi

Yuiko Kinose

Yoshihisa Harada

Daiki Nukaya

Fumitaka Osakada

Susumu Oshima

Sunao Sugita

Yoshiki (deceased) Sasai

Kiyotoshi Sekiguchi

The present invention provides a method for producing neural cells or a neural tissue, including the following steps (1)-(3): (1) a first step of culturing pluripotent stem cells in the absence of feeder cells and in a medium containing 1) a TGFβ family signal transduction pathway inhibiting substance and/or a Sonic hedgehog signal transduction pathway activating substance, and 2) a factor for maintaining undifferentiated state,(2) a second step of culturing the cells obtained in the first step in suspension to form a cell aggregate, and(3) a third step of culturing the aggregate obtained in the second step in suspension in the presence or absence of a differentiation-inducing factor to obtain an aggregate containing neural cells or a neural tissue.

Production method for nerve tissue

The present invention provides a method for producing retinal cells or a retinal tissue, comprising the following steps (1)-(3):

Production method for retinal tissue

An object of the present invention is to provide a cell population suitable for transplant of retinal tissue and a method of production thereof. The present invention provides a cell population for transplant, comprising retinal cells with a modified bipolar cell-regulating gene and a method of production thereof.

Human cell population comprising human retinal progenitor cells without an ISL1 gene for transplantation and methods of use thereof

The present invention aims to provide a method for suppressing differentiation of ganglion cell, amacrine cell, horizontal cell and/or bipolar cell in a neural retina tissue containing photoreceptor precursor and/or photoreceptor, and the like. A method for suppressing differentiation of a ganglion cell, an amacrine cell, a horizontal cell and/or a bipolar cell in a neural retinal tissue containing a photoreceptor precursor and/or a photoreceptor, including a step of culturing a retinal tissue comprising a neural retinal progenitor cell and in any stage between a differentiation stage immediately after emergence of a ganglion cell and a differentiation stage where emergence rate of a cone photoreceptor precursor reaches maximum in a medium containing a thyroid gland hormone signal transduction pathway agonist.

Method for producing retinal tissues

A cell population comprising Corin- and/or Lrtm1-positive cells was produced by the following steps (1) and (2), from which Corin positive and/or Lrtm1 positive cells are collected using a substance that binds to Corin and/or a substance that binds to Lrtm1, and dopaminergic neuron progenitor cells are produced by performing suspension culture of the Corin positive and/or Lrtm1 positive cells in a culture solution containing one or more nutritional factors:

Method for producing dopaminergic neuron progenitor cell

A sphere-like cell aggregate according to one embodiment of the present invention comprises: a core part containing neural retina; and a covering part continuously or discontinuously covering at least a portion of a surface of the core part.

Cell aggregate including retinal tissue and production method therefor

The invention relates to a method of producing a retinal pigment epithelial cell from a human pluripotent stem cell, and a method of treating or preventing a retinal disease by using the produced cell. The retinal pigment epithelial cell is prepared by (a) inducing differentiation of a human pluripotent stem cell into a pigment cell by adhesion cultivation of a human pluripotent stem cell in a medium containing a Nodal signal inhibitor and a Wnt signal inhibitor in the absence of a feeder cell to give a culture containing the pigment cell, (b) subjecting the obtained culture to further adhesion culture to give a culture containing a pigment cell colony, and (c) isolating the pigment cell from the obtained culture and culturing the cell to give a retinal pigment epithelial cell.

Method of producing human retinal pigment epithelial cells

The present invention provides a method for producing a retinal cell or a retinal tissue, including the following steps (1)-(3):

Method for producing retinal tissue

The present invention provides a method for producing neural cells or a neural tissue, including the following steps (1)-(3):

The present invention provides a method of producing a cell sheet comprising a retinal pigment epithelial cell layer and a vascular forming cell layer composed of stem cells having vascular formation ability or blood vessel constituting cells, comprising a step of laminating the retinal pigment epithelial cell layer and the vascular forming cell layer, and a cell sheet obtained by said method. Furthermore, the present invention provides a cell sheet for transplantation, comprising a cell layer formed with retinal pigment epithelial cells obtained by inducing differentiation of stem cells or progenitor cells ex vivo, a basement membrane secreted from said cells, and a vascular forming cell layer composed of stem cells having vascular formation ability or blood vessel constituting cells.

Method for producing retinal pigment epithelial cell sheet

The present invention provides an agent for treating ophthalmic diseases and a screening method for an agent for treating ophthalmic diseases and the like. The present invention also provides a method for predicting rejection associated with transplantation of retinal pigment epithelial cell to patients with ophthalmic diseases.

Eye disease treatment agent, screening method therefor, and method for predicting rejection response associated with retinal pigment epithelial cell transplant

The present invention provides a method of producing a cell sheet including the following steps (1) seeding and culturing retinal pigment epithelial cells on a collagen gel to form a cell sheet composed of the retinal pigment epithelial cells, and (2) degrading the collagen gel with collagenase to detach the cell sheet composed of the retinal pigment epithelial cells, and the like.

Method of producing retinal pigment epithelial cell sheet

A medical instrument includes a handpiece, a tank portion for housing a fluid, a plunger that changes a volume or pressure of an inside of the tank portion to suction the fluid into the tank portion or discharge the fluid from the tank portion, and an operating unit for operating the plunger. The handpiece includes a grip portion at the front end, and an operation member of the operating unit is disposed on a side of the grip portion.

Growing community of inventors

Wako, Japan

Masayo Takahashi