Hitachi, Ltd.

Hitachi-high-technologies Corporation

Applera Corporation

Applied Biosystems, LLC

Hitachi Instrument Engineering Co., Ltd.

Life Technologies Corporation

Masaya Kojima

Satoshi Takahashi

Ryoji Inaba

Tomohiro Shoji

Muneo Maeshima

Yasushi Shimizu

Yoshiyuki Okishima

Toshiaki Kita

Tomonari Morioka

Daizo Tokinaga

Miho Ozawa

Akihiro Suzuki

Shuhei Yamamoto

Eric S Nordman

Motohiro Yamazaki

Kazumichi Imai

Atsushi Ninomiya

Seiichi Ugai

Hiromi Yamashita

Masao Fukunaga

Takayasu Furukawa

Kiyoshi Tsukada

Shozo Kasai

Tomoyuki Sakai

Sadao Minakawa

Wataru Matsuo

Isamu Takekoshi

Yoshitaka Kodama

Taisaku Seino

Tomihiro Shoji

Masaru Enomoto

Takehiko Shibasaki

Syouzo Kasai

Ryouji Inaba

An electrophoresis apparatus includes a multi-capillary array having a liquid or solid disposed between the capillaries of the array. The liquid or solid exhibits a refractive index higher than that of air and less than that of water and reduces the amount of laser beams scattered by the capillaries. Also provided are methods of adjusting refracted and reflected excitation light beams passing through capillaries of a multi-capillary array, to reduce loss of intensity of the laser beams and increase irradiation of respective samples disposed in the capillaries.

Multi-capillary array electrophoresis device

A capillary array includes a light detection portion, a sample supply portion, a buffer solution supply portion and a voltage application portion which are necessary functions for electrophoresis, thereby, when assembling the capillary array into an electrophoresis apparatus, the same can be immediately used. Accordingly, a capillary array is provided which can be easily incorporated into an electrophoresis apparatus.

Capillary array

The troublesomeness during the setting of a plurality of capillaries is eliminated by composing pairs of electrodes, which are electrically connected to the common electrode, and capillaries. By bringing electrodes installed in the vicinity of each capillary disposed at the pitch of wells on the side of sample plate (within the area of the wells) into electrical contact with a common electrode, the capillaries and electrodes are made integral in construction. When a voltage is applied to the electrophoretic instrument via a common electrode portion, the voltage is applied to the electrodes for each capillary. This enables an inexpensive microtiter plate, etc. to be used and a multiple of capillaries to be simultaneously inserted, attached and detached.

Capillary electrophoretic instrument and capillary array assembly

Scattering on analysis in a multi-capillary electrophoresis apparatus is suppressed. The multi-capillary electrophoresis apparatus has a multi-capillary array that has a sample and a separation medium for separating a sample charged therein, and has a sampling section formed at ends of the capillaries and a detector part for acquiring information depending on the sample thus separated, a means for applying a voltage between the sampling section and the detector part, a chamber part having an air blowing mechanism and a temperature controlling mechanism, a capillary housing base provided on a leeward side of the air blowing mechanism and housing the multi-capillary array, and a first straightening plate provided between the chamber part and the capillary housing base.

Multi-capillary electrophoresis apparatus

At a wall constituting a space for a thermostatic oven in an electrophoresis apparatus, a capillary array attachment portion is formed which permits attachment of a plurality of capillary arrays having different length. Thereby, a selected capillary array constituted by collecting a plurality of capillaries can be easily attached to the electrophoresis apparatus depending on measurement purpose.

Capillary array electrophoresis apparatus and method of separating and analyzing specimen

A multi-capillary electrophoresis apparatus is provided for reducing errors during analysis caused by fluctuations in electrophoresis time among plural capillaries of the apparatus. The multi-capillary electrophoresis apparatus can contain a multi-capillary array that has a separation medium filled therein for isolating a sample. A detector component can be provided at a position remote from a sample injecting end of the array for acquiring information from the sample. A buffer container can be provided for holding a buffer solution into which the sample injecting end can be immersed. One or more temperature controlling component devices can be arranged for controlling a temperature of the buffer solution, of the detector component, or of both the buffer solution and the detector component.

A method of electrophoresis, an electrophoresis apparatus and a capillary array in which a fluorescently labelled sample is supplied into capillaries in a capillary array constituted by a plurality of capillaries and including a sample supply portion, an electrophoresis medium supply portion and a detection portion while controlling the temperature of the capillary array through gas circulation, the sample is caused to migrate and separated in the capillaries through electrophoresis, and at the region where the capillaries are contacted or come close each other a plurality of capillaries are contacted to a solid body so as to dissipate heat generated from the capillaries to the solid body.

Method of electrophoresis, electrophoresis apparatus and capillary array

An electrophoresis apparatus is provided in which negative effects caused by abnormalities in a current-carrying path in an electrophoresis apparatus can be avoided or reduced. The current flowing in the current-carrying path during electrophoresis can be measured to detect the state of a separating medium, and the application of a voltage to the current-carrying path can be interrupted.

Capillary electrophoresis apparatus and method

An electrophoresis device is disclosed that is capable of reducing waves occurring in a fluid in which the capillary tips of a capillary assay are immersed. A plurality of partition plate members can be provided in a container containing the fluid to divide the surface of the fluid into several sections. The container can be transported at a high speed, and the capillary tips can be immersed in the fluid quickly, thereby preventing resolution degradation caused by a long period of exposure of the capillary tips to air. Throughput of sample analysis can also be improved because it is not necessary to wait until waves disappear before conducting an analysis.

Capillary electrophoresis device

The capillary electrophoresis device using one pressure-resistant syringe () is capable of automatically suctioning in polymer solution and injecting polymer solution into a capillary array (). An electric hook () is installed for pulling up the syringe plunger on a push-in action type syringe (). A check valve () is inserted between a polymer bottle () and syringe to prevent solution from flowing back into the polymer bottle. As a result, one syringe portion of polymer solution can be suctioned and injected, a large volume of polymer solution can be continuously used and device processing capability is improved.

A multi-focus capillary array that can reduce crosstalk and a capillary array photodetector are provided. On a substrateon which a plurality of capillaries are aligned, a perforationis formed in at least a part of an area in the rear of the capillaries when the capillary array is viewed from the photodetector.

Capillary array and capillary array photodetector

Growing community of inventors

Mito, Japan

Masaya Kojima