Sysmex Corporation

Masatsugu Ozasa

Masanori Kawano

Yousuke Tanaka

Junya Inoue

Mitsumasa Sakamoto

Yasuyuki Kawashima

Shota Tateyama

Masakazu Fukuda

Hiroyuki Kobayashi

Keisuke Tsutsumida

Kunio Ueno

Hideki Hirayama

Takamichi Naito

Tatsuya Kosako

Rumi Takata

Kanako Nagaoka

Akinori Kawai

Kenji Hanamura

Takaaki Okajima

Daiki Mita

Daiki Fujiwara

A detection method according to one or more embodiments may include transporting a rack holding a reaction vessel along a first axis toward a sample dispensing position, linearly moving the sample dispensing pipette above the rack along a second axis intersecting the first axis and dispensing a sample into the reaction vessel located at the sample dispensing position, transporting the rack along the first axis toward a reagent dispensing position; linearly moving a reagent dispensing pipette above the rack along a third axis intersecting the first axis, and dispensing a reagent into the reaction vessel located at the reagent dispensing position, and detecting a detection target in a measurement specimen prepared from the sample and the reagent.

Detection method and detection device

Disclosed is a method for analyzing atypical cells in urine including: mixing urine, a diluent containing a surfactant, and a nucleic acid staining reagent to prepare a measurement specimen; irradiating the measurement specimen with light to detect scattered light and fluorescence light emitted from cells whose nucleic acids are stained; and detecting atypical cells contained in the measurement specimen distinguishably from white blood cells depending on a first characteristic parameter based on the scattered light and a second characteristic parameter based on the fluorescence light.

Method for analyzing atypical cells in urine, urine analyzer, and method for analyzing atypical cells in body fluid

A urine specimen analysis device includes a specimen drawing portion, a sample preparing portion, a measurement portion, and an information processing portion. The specimen drawing portion draws a first aliquot and a second aliquot from a urine specimen. The sample preparing portion prepares a first measurement sample by mixing the first aliquot and a first staining dye that stains red blood cells, and a second measurement sample by mixing the second aliquot and a second staining dye that stains nucleic acids. The measurement portion measures fluorescence emitted from the first measurement sample prepared by the sample preparing portion, and fluorescence emitted from the second measurement sample prepared by the sample preparing portion. The information processing portion detects at least red blood cells contained in the first measurement sample based on the fluorescence of the first measurement sample measured by the measurement portion, and at least white blood cells contained in the second measurement sample based on the fluorescence of the second measurement sample measured by the measurement portion.

Urine specimen analysis device and urine specimen analysis method

Disclosed is a urine sample analyzer for analyzing particles contained in a urine sample and outputting analytical results. The analyzer includes a flow cell that accepts a measurement specimen, the measurement specimen comprising a urine sample mixed with a reagent, a light irradiation unit positioned to irradiate the flowing measurement specimen with light, a light detector that detects light from individual particles in the flowing measurement specimen, and a data processor that receives signal from the light detector, processes the signal to obtain parameter information corresponding to a length of a cell cluster, and classifies fungi in the measurement specimen into groups by using the parameter information.

Urine sample analyzer and urine sample analyzing method

Disclosed is a urine specimen analyzing method which improves the detection of casts in a urine specimen by flowing a measurement sample containing a urine specimen through a flow cell, irradiating light on the measurement sample flowing through the flow cell, generating a signal waveform indicating a temporal change of intensity of light given off by the measurement sample, and detecting casts distinguishably from mucus threads contained in the urine specimen, based on information related to respective slope at both end sides of the signal waveform corresponding to each formed element contained in the urine specimen.

Urine specimen analyzing method, urine analyzer and non-transitory computer-readable storage medium

Disclosed is a sample analyzer which comprises: a sample preparing section configured to prepare a measurement sample by mixing a sample and a nucleic acid staining reagent; an optical detector configured to irradiate light on cells contained in the measurement sample, receive fluorescent light given off by the irradiated cells, and output fluorescent light signals; a signal processing section which obtains fluorescent light intensity and fluorescence pulse area of the cells from the fluorescent light signals output by the optical detector; and an information processing section configured to detect white blood cells contained in the measurement sample based on the fluorescence pulse area, and detect bacteria contained in the measurement sample based on the fluorescent light intensity.

Sample analyzer and sample analyzing method

Disclosed is a urine sample analyzing method comprising: flowing a measurement specimen prepared by mixing a urine sample and reagent through a flow cell; irradiating epithelial cells in the measurement specimen flowing through the flow cell with linearly polarized light and thereby producing scattered light; detecting a change of polarization condition of the scattered light produced by each of the epithelial cells; and classifying the epithelial cells into at least two types based on the change of polarization condition.

Urine sample analyzing method and sample analyzer including classifying epithelial cells into at least two types based on the change of polarization condition

Disclosed is a sample analyzing method comprising: flowing a measurement specimen prepared by mixing a sample and reagent through a flow cell; irradiating particles in the measurement specimen flowing through the flow cell with linearly polarized light and thereby producing scattered light; detecting a change of polarization condition of the scattered light produced by the particles; and discriminating erythrocytes from crystals in the measurement specimen based on the change of polarization condition.

Sample analyzing method and sample analyzer

An apparatus, intended for use in analyzing particles in urine is disclosed, that comprising: a sample distribution section for distributing urine samples to a first aliquot and a second aliquot; a first specimen preparing section for preparing a first specimen for measuring urinary particles, containing at least erythrocytes, by mixing a first stain reagent and the first aliquot; a second specimen preparing section for preparing a second specimen for measuring bacteria by mixing a second stain reagent and the second aliquot; and an optical detecting section comprising a light source for irradiating a light to a specimen being supplied, a scattered light receiving element for detecting scattered light emitted from the specimen, and a fluorescence receiving element for detecting fluorescence emitted from the specimen. A method intended for use in analyzing particles in urine is also disclosed.

Apparatus for analyzing particles in urine and method thereof

A particle analyzing apparatus, comprising: a flow cell which forms a specimen flow including particles; first and second light sources; an irradiation optical system which applies lights emitted from the first and second light sources so that the lights are applied to the specimen flow; a detector which detects forward scattered light, the forward scattered light being emitted from the first light and scattered by the particle in the specimen flow, and generates a signal according to the detected scattered light; a light blocking member disposed between the flow cell and the detector; a controller which obtains characteristic parameters of the particle based on the signal from the detector; and an imaging device which captures an image of the particle in the specimen flow using the light from the second light source is disclosed. Particle imaging method is also disclosed.

Particle analyzing apparatus and particle imaging method

An analyzer comprises: a sample stream forming section for forming a sample stream including particles; a light source; an optical fiber bundle formed by a plurality of optical fibers, into which light from the light source enters, and which emits light onto the sample stream; and an imaging device for imaging a particle in the irradiated sample stream.

Analyzer and particle imaging method

An apparatus for analyzing particles in urine comprising is disclosed that includes: a measurement specimen preparing section for preparing a measurement specimen by using a urine sample and a stain reagent; an optical detecting section comprising a light source for emitting a light to the prepared measurement specimen, a forward-scattered light receiving element for detecting forward-scattered light emitted from the specimen, a side-scattered light receiving element for detecting side-scattered light emitted from the specimen, and a fluorescence receiving element for detecting fluorescence emitted from the specimen; and a measurement section for measuring leukocytes in urine, based on the forward-scattered light, the side-scattered light and the fluorescence detected by the optical detecting section. A method for analyzing particles in urine is also disclosed.

A sheath liquid for use with a particle analyzer has a refractive index of 1.3340 or more at the wavelength of sodium D line at 25&deg; C.

Sheath liquid for particle analyzer

A flow cytometer includes a flow cell for flowing a sample liquid in a flowing direction, to form a sample flow the sample liquid containing particles to be analyzed, a laser diode radiating a laser beam having an elliptic cross section, a beam collimating section for collimating the laser beam radiated from the laser diode, a beam spot forming section for focusing the collimated beam at the sample flow in the flow cell to form a beam spot, and a light receiving section for receiving light generated from the particles at the beam spot to detect optical information of the particles, wherein the laser diode is arranged such that a minor diameter of the elliptic section of the laser beam is parallel to the sample flow.

Growing community of inventors

Kobe, Japan

Masatsugu Ozasa