Oriental Yeast Co., Ltd.

Masatsugu Nonobe

Tsuyoshi Fujita

Hozumi Nishida

Tetsuo Kitahara

Kouji Sugita

Tsuyosi Fujita

Hozumi Hamasaki

An accurate and effective measurement of copper in subject samples is rendered possible even for minute amounts of copper, by converting copper-bonded hologalactose oxidase to the apo-form by liberation of the copper, and measuring the copper of the sample in a reaction system containing the apogalactose oxidase.

Enzymatic methods for measurement of minute amounts of copper

Calcium in a sample is brought into contact with a transglutaminase capable of being activated with calcium as an activating factor and the transglutaminase activity, which varies depending upon the calcium amount in the sample, is measured to thereby determine the calcium amount in the sample. By the method of the invention, accurate determination of calcium in various samples such as body fluids is possible without removal of proteins from them.

Method of determination of calcium

Crystalline potassium salt of thinonicotinamide adenine dinucleotide

Assay method and assay reagent for serum iron or unsaturated iron

Growing community of inventors

Hyogo, Japan

Masatsugu Nonobe