National Institute of Advanced Industrial Science and Technology

Konica Minolta, Inc.

Masatoshi Kataoka

Shohei Yamamura

Shouki Yatsushiro

Yasunori Chiba

Yoshihiro Nakajima

Yusuke Fuchiwaki

Jungo Araki

Kenichi Nakayama

Hiroko Abe

Kumiko Hoshi

Kazuya Tomimoto

Yasuko Fujita

Toshihiko Ooie

Tomoko Iwaki

Hiroki Takaoka

A cell-spreading device may include a microchamber chip having a microchamber capable of enclosing and retaining a cell, a channel-forming frame united with the microchamber chip to form a channel on the microchamber, an inlet provided in the channel-forming frame to allow a cell suspension to flow into the channel, and an outlet provided in the channel-forming frame to allow the cell suspension, which has been allowed to flow into the channel through the inlet, to flow out from the channel. When an aperture of the microchamber is projected perpendicularly to a longitudinal width of the microchamber chip, the void ratio that is a ratio of the sum total of voids to the longitudinal width is not more than 5%, the void being a length of a portion where the projected aperture of the microchamber is not present against the longitudinal width.

Cell-spreading device and method for detecting rare cell

The assay device () comprises a micro flow path (); a porous medium provided near the distal end portion () of the micro flow path (); and a space () provided between the micro flow path () and the porous medium for controlling the flow rate of a fluid moving from the micro flow path () to the space (). After a fluid moved along the micro flow path () based on a lateral flow is brought into contact with the porous medium beyond the space () and is absorbed to the porous medium, the fluid is divided by the space () so that the fluid stays in the micro flow path (). With this structure, it is possible to perform solution exchange in the micro flow path without using an external device such as a pump.

Assay device using porous medium

Disclosed is a detection method for detecting a specific cell in a sample containing multiple cells including the specific cell. Specifically disclosed is a detection method for detecting a specific cell in a sample containing multiple cells including the specific cell, which comprises the following steps (1) and (2): (1) retaining cells contained in the sample on a microarray chip which comprises multiple microchambers, wherein each of the microchambers can contain multiple cells; and (2) confirming the presence or absence of the specific cell in the cells retained on the microarray chip.

Cell detection method, and microarray chip for use in the method

The present invention provides: genetically modified yeasts such as mutant yeasts having an ability to produce N-linked sugar chains of ManGlcNAcand a decreased ability to produce O-linked sugar chains, mutant yeasts having an ability to produce N-linked sugar chains of ManGlcNAcand further having an ability to produce N-linked sugar chains of GlcNAcManGlcNAc, and mutant yeasts having an increased ability to produce and secrete proteins and an ability to produce N-linked sugar chains of ManGlcNAc; and a method for producing glycoproteins using them.

Sugar-chain modified yeast and method for producing glycoprotein using the same

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Takamatsu, Japan

Masatoshi Kataoka