Medinet Co., Ltd.

Masato Muto

Mie Nieda

Mai Tomiyama

Disclosed are: a method for culture of disease antigen specific CTLs and &#x3b3;&#x3b4;T cells in one culture step conveniently and efficiently; and a pharmaceutical agent and a therapeutic/prophylactic method both of which use a cell produced by the method. Blood is collected and peripheral blood mononuclear cells are separated from the blood. Aminobisphosphonate and a disease antigen are added to the peripheral blood mononuclear cells at the beginning of culture, and the cell culture is carried out for a predetermined period to proliferate/induce disease antigen specific CTLs and &#x3b3;&#x3b4;T cells simultaneously until the numbers of the cells reach values that are effective for the treatment of a disease. The CTLs and the &#x3b3;&#x3b4;T cells thus produced are used for the treatment.

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Tokyo, Japan

Masato Muto