Takeda Chemical Industries, Inc.

Masato Kuriyama

Osamu Nishimura

Tsunehiko Fukuda

Nobuyuki Koyama

Kazuaki Kitano

Shigeru Morita

Kazutaka Maejima

A cysteine-free peptide is produced by producing a fused protein comprising a protein having cysteine at its N-terminal and a cysteine-free peptide ligated to the N-terminal and subsequently subjecting the fused protein to a reaction for cleaving the peptide linkage.

Method for producing a biologically active recombinant cysteine-free peptide

Method for producing a biologically active recombinant cysteine-free

There are disclosed cloning of an alkaliprotease secretion gene of a mold and a DNA fragment containing a part or all of the alkaliprotease gene. A plasmid into which the DNA fragment is integrated, a transformant obtained by using the plasmid and the production of a useful protein by using the transformant are also disclosed.

DNA coding for alkaline protease

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Osaka, Japan

Masato Kuriyama