Osaka University

Quantum Biosystems Inc.

Shin-etsu Chemical Co., Ltd.

Aipore Inc.

Kabushiki Kaisha Toshiba

Tokyo Institute of Technology

Tokyo Medical and Dental University

Masateru Taniguchi

Tomoji Kawai

Makusu Tsutsui

Kazumichi Yokota

Ikuo Fukui

Takahito Ohshiro

Takashi Washio

Mark F Oldham

Eric S Nordman

Wataru Tonomura

Soh Ryuzaki

Hideyuki Kawaguchi

Eriko Mizuno

Takeshi Yoshida

Akihide Arima

Masakazu Sanada

Tadashi Miyagi

Kohji Nishida

Masayoshi Tanaka

Yoshinobu Baba

Sadato Hongo

Takao Yasui

Yuji Miyahara

Noritada Kaji

Noriyasu Hashida

Mina Okochi

Akira Ishii

Akira Ishi

Yukichi Horiguchi

A current measurement method for measuring a tunneling current in biopolymers passing through between a pair of electrodes includes arranging the electrodes in a liquid that contains an electrolyte and, while applying a voltage between the electrodes, measuring a current flowing between the electrodes via an electric double layer formed along surfaces of the electrodes. This enables measuring the current in consideration of the electric double layer. As a result, it is possible to more accurately measure the tunneling current in the biopolymers included in a liquid sample that contains an electrolyte.

Current measurement method

Provided are a virus measuring method, a virus measuring device, a virus determining program, a stress determining method, and a stress determining device. A virus measuring method includes a contact step of bringing a liquid specimen containing a body fluid of a subject and an electrolytic solution into contact with each other via a through-hole portion formed in a separating wall, a current measuring step of applying a voltage to the liquid specimen and the electrolytic solution with respect to the through-hole portion and obtaining a waveform of an ionic current flowing through the through-hole portion, and a virus determining step of determining the kind of a virus contained in the body fluid on the basis of the waveform. In the virus determining step, the kind of the virus is determined by comparing the waveform with waveform information that corresponds to a known virus and is obtained beforehand.

Virus measuring method, virus measuring device, virus determining program,stress determining method, and stress determining device

A number analyzing method, a number analyzing device, and a storage medium for number analysis are disclosed, which enable, with high accuracy, analysis of the number or number distribution of particulate or molecular analytes according to the kinds of the analytes. A computer control program is executed on the basis of a data group of particle-passage detection signals which are detected by a nanopore device in accordance with passage of subject particles through a through-hole. Also, a particle type distribution estimating program is executed, to estimate probability density on the basis of a data group based on feature values indicating feature of the waveforms of pulse signals which correspond to the passage of particles and which are obtained as the particle-passage detection signals. Thus, the number of particles can be derived for each particle type.

Number analyzing method, number analyzing device, and storage medium for number analysis

Provided is a channel device that is capable of increasing the concentration of fine particles in a liquid only by use of fluid-dynamic flows without relying on electrostatic interactions. A channel device () in accordance with an embodiment of the present invention includes: a main channel () configured to allow a liquid containing fine particles to flow therethrough; a chamber () that is provided at an end of the main channel () and that is configured to store target fine particles which have increased in concentration; and a side channel () that is connected to a side face of the main channel () and that is configured to allow unwanted liquid to drain therethrough, wherein at least one of a height and a width of the side channel () is smaller than a particle size of the fine particles.

Channel device and method for concentrating fine particles

It is an object to improve detection accuracy of an object as compared with prior arts. A flow passage () provided in a detection device () includes a substrate () and a covering member () provided at a position corresponding to the substrate (). A covering member opening (HL) of the covering member () is provided such that a substrate opening (HL) of the substrate () is not covered with the covering member (). The covering member () is arranged onto the substrate () such that a substrate capacitance and a covering member capacitance are connected in series. The covering member capacitance is lower than the substrate capacitance.

Flow passage

A device for biological material detection includes a substrate; a through-hole through which a biological material to be tested passes, the through-hole being formed in the substrate; a molecule that interacts with the biological material to be tested passing through, the molecule being formed in the through-hole; a first chamber member that forms, with at least the surface including the through-hole on one surface side of the substrate, a first chamber to be filled with electrolyte; and a second chamber member that forms, with at least the surface including the through-hole on the other surface side of the substrate, a second chamber to be filled with electrolyte. The biological material to be tested is identified by the waveform of the ion current (passage time, shape, etc.) when the biological material to be tested passes through the through-hole.

Device for biological material detection, detection apparatus for biological material detection, method for measuring ion current, and method for identifying biological material

The present invention provides technology that uses current measurements to identify nucleotides and determine a nucleotide sequence in polynucleotides. The present invention calculates a modal value of a tunnel current that arises when a nucleotide or polynucleotide for analysis passes through between electrodes, and then employs the calculated modal value. The present invention accordingly enables direct rapid implementation to identify nucleotides and to determine a nucleotide sequence in a polynucleotide without marking.

Method and device for identifying nucleotide, and method and device for determining nucleotide sequence of polynucleotide

Devices, systems and methods for sequencing protein samples are provided. In some examples, currents generated when a monomer passes through between electrodes of a nanogap electrode pair are measured for each of several different distances, so that monomers are identified when compared to a reference physical quantity of a known monomer, which may be obtained from a current measured with a similar inter-electrode distance(s) at which each of plural kinds of monomers are identifiable and ordered with predetermined accuracy and based on a detected physical quantity obtained from a tunneling current, which may be further normalized by the use of one or more reference substances.

Biomolecule sequencing devices, systems and methods

The present disclosure provides methods and structures for systems which can linearize and capture a nucleic acid molecule (e.g., DNA) for re-measurement of the nucleic acid molecule or other polymer prior to detection of the polymer. The structures may allow for quick exchange between different samples or other reagents.

Devices, systems and methods for linearization of polymers

According to one embodiment, provided is a single particle analyzing device including a measuring vessel, first and second chambers in the vessel defined by an insulating membrane, a pore opening in the membrane to connect the chambers, and first and second electrodes in the chambers. Electric current flows between the electrodes through the pore. Electrical characteristics are measured during migration of the target from the first chamber to the second chamber to measure the size and shape of the target. (a) t&#x3c;a &#x3c;d&#x2266;100or (b) s&#x3c;L, s&#x3c;d&#x2266;100, t&#x3c;L and t&#x3c;d, wherein a, L and s are the diameter, length and width of the target, d is the diameter of the pore, and t is the thickness of the membrane in the proximity to the pore.

Single particle analyzer and single particle analysis method

The analysis method allows analysis of samples with high sensitivity, irrespective of interelectrode distance. The method includes: a step of applying a voltage between a first electrode pair such that an electric field is formed in a direction intersecting a migration direction of a sample; a step of placing a solution, including an electrochemically active molecule that produces a redox reaction at the electrode pair, between the first electrode pair; a step of causing the sample to migrate; and a step of measuring an amount of change in current flow between the first electrode pair.

Sample analysis method

The present invention provides a method and apparatus for controlling the moving speed of a substance, both of which can adjust the moving speed of a substance to a desired speed. The control method and control apparatus cause a substance to pass through an internal space, in which an electro-osmotic flow is generated, of a surround electrode formed so as to surround part of the moving path of the substance, whereby the control method and control apparatus change the moving speed of the substance.

Method for controlling substance moving speed and apparatus for controlling the same

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Osaka, Japan

Masateru Taniguchi