Juridical Foundation the Chemo-sero-therapeutic Research Institute

Kumamoto University

Masatake Araki

Chikateru Nozaki

Hiroshi Yonemura

Ken-ichi Yamamura

Junichi Miyazaki

Kimi Araki

A method of producing a chimera animal, characterized by transplanting, into a host animal, an aggregate of a first pluripotent stem cell that is of interest to strain establishment, a second pluripotent stem cell in which the ability to form germ cells is absent or decreased, and embryo.

Method of establishing mouse strain

An expression vector for the expression of an exogenous gene in an animal cell, which contains a chick .beta.-actin gene promoter and a restriction enzyme site for incorporating an exogenous gene at the downstream of said promoter and a process for expressing an exogenous gene, which comprises incorporating said exogenous gene into the expression vector at the restriction enzyme site for incorporating the exogenous gene, introducing said vector into an animal cell and culturing the obtained transformed animal cell. The expression system of the present invention can be applied to an expression of any exogenous gene and has an extremely high expression efficiency and is applicable to a wide range of host cells, and hence can sufficiently be utilized for industrial-scale production of a useful material.

Exogenous gene expression vector containing chick .beta.-actin gene

Growing community of inventors

Kumamoto, Japan

Masatake Araki