Riken Corporation

Hokkaido University

Medical Biological Laboratories Co., Ltd.

Olympus Corporation

Olympus Optical Company, Ltd.

Japan Science and Technology Agency

Sysmex Corporation

Osaka University

National Institute of Information and Communications Technology, Incorporated

Masataka Kinjo

Atsushi Miyawaki

Satoshi Karasawa

Hiroshi Hama

Toshio Araki

Kenta Saito

Takako Kogure

Hirokazu Kubo

Shigehito Miki

Hirotaka Terai

Mamoru Tamura

Akshay Ganguly

Yasushi Hiraoka

Tokuko Haraguchi

Taro Yamashita

Motohiro Horiuchi

Fumihiko Fujii

Hiroshi Sakata

Masayoshi Ueno

Takayuki Yanagiya

Lausonia Ramaswamy

Disclosed is a method for acquiring information of a target polypeptide, comprising: acquiring a diffusion time of a fluorescently labeled target polypeptide and a diffusion time of each of a plurality of fluorescently labeled reference polypeptides by fluorescence correlation spectroscopy or fluorescence cross-correlation spectroscopy, and acquiring information on size of the fluorescently labeled target polypeptide from the diffusion time of the fluorescently labeled target polypeptide with reference to the diffusion times of the plurality of fluorescently labeled reference polypeptides, wherein information on size of each of the plurality of fluorescently labeled reference polypeptides is known, and the sizes of the plurality of reference polypeptides are different from each other.

Method for acquiring information of target polypeptide and reagent kit

Provided is a light detecting device including: a light path branching unit that branches a single detection light path of fluorescence from a specimen, into a plurality of branched light paths; a plurality of light detectors that are provided to the respective branched light paths branched by the light path branching unit and that include an SSPD or Geiger mode APD to detect the fluorescence; and a signal adder that generates a single image signal in accordance with the detection signals outputted from the plurality of light detectors.

Light detecting device and laser microscope system

An object of the present invention is to provide a red or orange fluorescent protein, which is characterized in that the difference (stokes shift) between an excitation peak value (wavelength of maximum absorption) and a fluorescence peak value (wavelength of maximum fluorescence) is greatened, so that the maximum fluorescence can be obtained by the maximum excitation. The present invention provides a novel fluorescent protein monomerized by introducing a mutation into a florescent protein derived fromsp., and a novel chromoprotein and fluorescent protein derived from. sp.

Fluorescent protein

The present invention provides a method of quickly and accurately detecting and/or assaying an antigen using fluorescence correlation spectroscopy (FCS), which involves a fluorescence-labeled antibody fragment and a non-fluorescence-labeled intact antibody that form a complex with the antigen. There is a significant difference in diffusion rate between the fluorescence-labeled antibody fragment not bonded to the antigen and the complex formed by the the fluorescence-labeled antibody fragment, the antigen, and the non-fluorescence-labeled intact antibody, and this diffusion rate can be determined using FCS. The antigen can be an antigenic protein, such as an abnormal prion or a harmful protein contained in a food material. According to this method, antigens over a wide scope can be assayed regardless of the shape or molecular weight.

Method of quickly detecting and/or assaying antigen by fluorescence correlation spectrometry

A biological sample containing a target nucleic acid is mixed with a primer, a substrate labeled with fluorescence, and DNA polymerase to prepare a test solution. After a nucleic acid amplification reaction is performed in a predetermined manner, the test solution is attached dropwise on a slide glass, which is mounted on a sample holder of an inverted fluorescence microscope. Data measured by a photomultiplyer are arithmetically operated by applying an autocorrelation function in a data processing apparatus. Based on the arithmetic results, quantification data for the target nucleic acid, are numerically or graphically displayed on a screen of a display apparatus.

Method of analyzing a target nucleic acid

Growing community of inventors

Sapporo, Japan

Masataka Kinjo