Chisso Corporation

Jnc Corporation

Masashi Ushiyama

Shigeyuki Aoyama

Yuichi Onji

Kongen Aoki

The object of the present invention is to provide a compound used in a reagent for detecting microorganisms, by which microorganisms can be rapidly and reliably detected without requiring any special instrument, the reagent for detecting microorganisms, and a method for detecting microorganisms. The object can be achieved by a tetrazolium compound represented by general formula (I) below: wherein the symbols are as defined in the description, a chromogenic reagent for detecting microorganism comprising the tetrazolium compound, or a method for detecting microorganisms using the chromogenic reagent.

Tetrazolium compound for detecting microorganisms, reagent for detecting microorganisms and method for detecting microorganisms

To provide a microorganism culture vessel that can control existence of invading foreign matters and put a large amount of sample therein, a microorganism test kit, a method for testing a dialysate, a method for culturing the microorganism, a method for testing the microorganism and a method for producing the microorganism culture vessel. The invention includes the microorganism culture vessel, including an inlet-equipped culture bag in which a sheet-like medium is put and sealed.

Microorganism culture vessel, microorganism test kit, method for testing dialysate, method for culturing microorganism, method for testing microorganism and method for producing microorganism culture vessel

A microorganism culture device comprising a porous matrix layer having a basis weight of 40 to 100 g/mand an air permeability of 7 to 24 cm/sec, and at least one layer of a water-soluble polymer layer laminated with the matrix layer.

Microorganism culture device and microorganism culture medium comprising the same

A microorganism culture medium comprising a derivative A' of a chromogen A, and a chromogen B, where the chromogen A is a chromogen being oxidized to a colorant, and the chromogen B is a chromogen being reduced to a colorant. The derivative A&#x2032; acts as a substrate of an enzyme generated when a microorganism is growing, and is then converted to the chromogen A, and the derivative A&#x2032; and the chromogen B are substantially not colored in the culture medium unless a microorganism is inoculated.

Microorganism culture medium

A material for culture media comprises a fiber layer; a polymeric compound layer A free of a color former and a selecting agent; and a polymeric compound layer B containing a color former and/or a selecting agent, which are put in layers in this order. The material permits the precise and rapid microbial test of subjects having a curvature or unevenness to some extent. In addition, the material can save space required for the test and can reduce the amount of waste generated after the practical use thereof.

Culture medium for microorganisms

A culture material is formed containing a water-soluble polymer layer and a porous matrix layer arranged such that when water is applied to the surface of the matrix layer the water diffuses through the matrix layer to dissolve the water-soluble polymer layer which then gradually penetrates into the matrix layer to form a single layer for use as a culture medium on which a microorganism can be grown to form colonies. The water-soluble polymer layer or water added to the matrix layer can contain nutrients for the microorganism, and the microorganism can be added with the water. The culture material has a water content of not more than 50%, and is sufficiently insoluble in water such that it substantially holds its original shape for at least 30 minutes when in contact with water having a temperature of not more than 50&deg; C. The culture medium produced by adding water to the culture material may be used for quantitative or semi-quantitative determination of microorganisms that may be present in a sample such as obtained from food.

Growing community of inventors

Yokohama, Japan

Masashi Ushiyama