Juridical Foundation the Chemo-sero-therapeutic Research Institute

The Chemo-sero-therapeutic Research Institute

Km Biologics Co., Ltd.

Masashi Sakaguchi

Eiji Tokunaga

Ryuichi Sakamoto

Fukusaburo Hamada

Toshihiro Ushijima

Kazuo Matsuo

Sachio Tokiyoshi

Michitaka Yamamoto

Hiroaki Maeda

Hiroshi Mizokami

Junichi Miyazaki

Kenji Yokogawa

Kozo Takase

Susumu Baba

Takashi Imamura

Kengo Sonoda

Kenichi Yamazaki

Hideyuki Ohta

A vaccine composition for birds comprising as an active ingredient a structure containing O-antigen derived from Gram-negative bacteria, provided that said structure does not contain a whole cell, and a process for preparing the same are provided. By using a structure containing O-antigen (e.g. lipopolysaccharide) derived from Gram-negative bacteria as an active ingredient in accordance with the present invention, alleviation of inoculation reaction and reduction in an amount of injection are attained as compared to the conventional whole-cells vaccine to thereby allow for the increase in the number of other antigens to be mixed therewith.

LPS vaccine

A recombinant avian infectious coryza vaccine and a process for preparing the same are provided. A process for preparing a recombinant avian infectious coryza vaccine which comprises step of constructingthat may produce as an inclusion body a fusion peptide consisting of peptides derived from outer-membrane protein ofserotype A and serotype C, step of culturing saidand collecting and purifying inclusion body from culture, and step of preparing a preparation comprising said purified inclusion body, and an avian infectious coryza vaccine comprising as an active ingredient the fusion peptide. A linker sequence may be inserted between the respective peptides comprising the fusion peptide. For the peptide derived from the serotypes A and C, an amino acid sequence region of Region 2 or its vicinity responsible for protection from infection may be used.

Recombinant avian infectious coryza vaccine and process for preparing same

A variant ofsurface protective antigen SpaA protein or of a shortened form of SpaA (&#x394;SpaA) in which a portion of SpaA protein is deleted for protection frominfection and a process for preparing the same are provided. Introduction of amino acid substitution at a specific site in the amino acid sequence of SpaA or &#x394;SpaA protein provides a variant of SpaA or &#x394;SpaA protein which is immunogenic and is expressed inas inclusion bodies. The variant of SpaA or &#x394;SpaA protein of the present invention may easily be recovered and purified since it is expressed inas inclusion bodies.

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A method and a kit for detecting an antibody toare provided. A method for detecting an antibody towhich comprises detecting an antibody induced by an outer-membrane protein ofserotype A and/or serotype C by ELISA with a solid phase to which a peptide consisting of an amino acid sequence of non-homologous region of said outer-membrane protein or a portion thereof is immobilized, and a detection kit used for said method.

Method and kit for detecting antibody to avibacterium paragallinarum

A novel peptide obtained fromhas been found useful for preventing avian infectious coryza. This polypeptide induces production of hemagglutination-inhibition antibody and prevents infection and onset of avian infectious coryza. The invention further provides a gene coding for the polypeptide, a recombinant vector for expression of this gene, a host transformed with this vector, a process for preparing the polypeptide in a host, a vaccine for avian infectious coryza comprising the polypeptide as an active ingredient, a monoclonal antibody obtained using the polypeptide as an immunogen, and a diagnostic agent and a therapeutic agent for avian infectious coryza using the peptide and the antibody.

Polypeptide for Haemophilus paragallinarum and process for preparing the same

A novel peptide obtained from has been found useful for preventing avian infectious coryza. This polypeptide induces production of hemagglutination-inhibition antibody and prevents infection and onset of avian infectious coryza. The invention further provides a gene coding for the polypeptide, a recombinant vector for expression of this gene, a host transformed with this vector, a process for preparing the polypeptide in a host, a vaccine for avian infectious coryza comprising the polypeptide as an active ingredient, a monoclonal antibody obtained using the polypeptide as an immunogen, and a diagnostic agent and a therapeutic agent for avian infectious coryza using the peptide and the antibody.

Polypeptide originating in haemophilus paragallinarum and process for producing the same

The preset invention provides a method for immunizing chickens which comprises inoculating into growing egg a composition comprising either cell-free attenuated viruses of Marek's disease type 1 or cells infected with attenuated viruses of Marek's disease type 1 capable of producing cell-free viruses. The present invention also provides a method for immunizing chickens which comprises inoculating into growing egg a mixed vaccine comprising said composition plus another vaccine from at least one microorganisms selected from the group consisting of viruses other than virus of Marek's disease type 1, bacteria and protozoan.

In ovo vaccination of marek's disease type I virus

Recombinant Marek's disease virus (MDV) and vaccine

A recombinant Marek's disease virus produced by the mutation of a Marek's disease virus with a plasmid wherein said plasmid comprises (1) a gene fragment derived from the Us region or inverted repeat sequences adjacent to both ends of said Us region of a Marek's disease virus genome and (2) an exogenous gene expression cassette incorporated in said gene fragment, said cassette comprising an exogenous gene bound downstream of a promoter derived from an animal cell or an animal virus, a process for preparing the same, a multivalent live vaccine for birds comprising the same, and a vector for administration of a physiologically active substance to birds which comprises the same.

Recombinant Marek's disease virus, process for preparing the same and

A recombinant Marek's disease virus which comprises a Marek's disease virus genom and a DNA fragment incorporated therein, said DNA fragment being constructed by incorporating a promoter derived from an animal cell or an animal virus and a structural gene coding for an exogenous protein into a gene fragment derived from a Marek's disease virus, a process for preparing the same which comprises preparing a gene fragment wherein a structural gene coding for an exogenous gene is linked to the downstream of a promoter derived from an animal cell or an animal virus, incorporating said gene fragment into a BamHI - H fragment of a gene of a Marek's disease virus type I, and incorporating said fragment into a Marek's disease virus type I genome, and a multivalent live vaccine for birds comprising the same.

Growing community of inventors

Kikuchi, Japan

Masashi Sakaguchi