Nitto Chemical Industry Co., Ltd.

Nitto Kagaku Kogyo Kabushiki Kaisha

Masaru Suto

Hitoshi Shimizu

Takeyuki Fujita

Susumu Seki

Kazumoto Ogura

Koichiro Ryuno

Takanori Fujimoto

A method for the cultivation of bacteria of the genus Pseudomonas capable of producing nitrile hydratase is disclosed. The method involves adding a water soluble copper compound in an amount of about 0.5 to 5.0 mg/l as calculated in terms of copper to a culture medium in the preparation of cells of the bacteria under shear force supplied by stirring blades, wherein the circumferential speed of the edges of the stirring blades exceeds about 2.5 m/sec. The water soluble copper compound is preferrably copper chloride, copper sulfate, copper nitrate, copper acetate, copper tartrate, copper (II) acetylacetonate or copper (II) ETDA.

Method for cultivation of nitrile hydratase-producing pseudomonas

A process for producing dimethylformamide which comprises reacting methanol and ammonia to give methylamines, separating a dimethylamine-trimethylamine mixture from the methylamines, and reacting the dimethylamine-trimethylamine mixture with carbon monoxide to produce dimethylformamide.

Process for production of dimethylformamide

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Yokohama, Japan

Masaru Suto