Genentech, Inc.

Masaru Ken Shiratori

Michael Kim

Robert David Kiss

Salim Charaniya

Justin Bourret

Hardayal Prashad

Raquel Iverson

Bradley Richard Snedecor

Michael W Laird

Shahram Misaghi

Tim Breece

Jordan James Baker

Nathaniel Thomas Klair

James Wilkins

The present disclosure relates to methods, cells and compositions for producing a product of interest, e.g., a recombinant protein. In particular, the present disclosure provides improved mammalian cells expressing the product of interests, where the cells (e.g., Chinese Hamster Ovary (CHO) cells) have modulated lactogenic activity. The present disclosure also relates to methods and compositions for modulating pyruvate kinase muscle (PKM) expression (e.g., PKM-1 expression) in a mammalian cell to thereby reduce or eliminate the lactogenic activity of the cell, as well compositions comprising a cell having reduced or eliminated lactogenic activity and methods of using the same.

Modulating lactogenic activity in mammalian cells

The invention provides for methods of viral inactivation using high temperature short time (HTST) treatment and adjustment of various parameters such that generation of precipitate and depositions of precipitate are reduced and/or minimized.

Methods for viral inactivation and other adventitious agents

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Burlingame, CA, United States of America

Masaru Ken Shiratori