Yamasa Corporation

Masaru Hamaoki

Seiji Tanaka

The present invention provides a method for determining the degree of macrophage-associated negative effects on a vertebrate including a human, the method including assaying diacetylpolyamine contained in a sample collected from the vertebrate. According to the method of the present invention, metabolic conditions of macrophages can be monitored, and the degree of macrophage-associated negative effects on a vertebrate (including a human) can be determined. Specifically, the present invention can predict, through assay of diacetylpolyamine, pathological condition which is considered a macrophage-related disease; e.g., recurrence of cancer or malignant tumor, or infiltration or activation of cancer or malignant tumor cells; denaturation or degeneration of neurons associated with Alzheimer's disease; or onset or progression of an autoimmune disease (e.g., rheumatism or Crohn's disease) or arteriosclerosis. Therefore, the present invention is very useful for clinical tests.

Method for determining degree of negative effect of macrophages on vertebrate

The present invention relates to a method for long-term stabilizing a pulmonary surfactant protein, to a stabilized aqueous solution containing a pulmonary surfactant protein, and to a kit for assaying a pulmonary surfactant protein which kit contains, as a component reagent, a stabilized aqueous solution containing a pulmonary surfactant protein. The invention provides a method for stabilizing a pulmonary surfactant protein, the method including causing the pulmonary surfactant protein to be present with a calcium ion and an oxidizing/reducing substance. The invention also provides an aqueous solution containing a pulmonary surfactant protein which has been stabilized by use of a calcium ion and an oxidizing/reducing substance in combination. The invention also provides a kit for assaying a pulmonary surfactant protein present in a sample through an immunological technique employing antigen-antibody reaction, the kit containing, as a standard solution of pulmonary surfactant protein, an aqueous solution containing a pulmonary surfactant protein which has been stabilized by use of a calcium ion and an oxidizing/reducing substance in combination.

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Choshi, Japan

Masaru Hamaoki