Shionogi Company Limited

Kyoto Daiichi Kagaku Co., Ltd.

Shionogi Seiyaku Kabushiki Kaisha

Masao Kono

Akira Yamauchi

Ken Inouye

Tadashi Okabayashi

Nobuo Yoshida

Shoichi Ishihara

Taichitro Komeno

Ken'ichi Igano

Xiaoming Dou

Akira Ueda

Toshio Takama

Masuhisa Nakamura

Yung Xiang Wang

Michio Ogawa

Atsushi Misaki

Mitsuhiro Okamoto

Kenichi Igano

Misao Ide

Tetsuo Tsuji

Akiko Kanda

A hybridoma producing a monoclonal antibody recognizing the C-terminus of human brain natriuretic peptide (hBNP) was cultivated in a medium or the abdominal cavity of a mouse to recover the monoclonal antibody from the medium or ascites accumulated in the abdominal cavity. An immunoassay for hBNP was established using the monoclonal antibody. The immunoassay for hBNP of the invention is so sensitive that the minimum detection limit is 1 pg/ml and can therefore determine the hBNP level in blood plasma directly, without the extraction of hBNP from blood plasma. It is useful for diagnosing diseases such as hypertension and the like, and states of the heart, kidney, and the like by using the increase/decrease of the hBNP level as an index.

Monoclonal antibody recognizing C-terminus of hBNP

A method for measuring the concentration of polynucleotide in a sample comprising the steps of: (1) preparing a sample, a Raman scattering active agent capable of bonding to a polynucleotide and a surface-enhanced Raman scattering (SERS) active substrate capable of capturing the agent; (2) mixing the sample, the agent and the substrate to capture the agent which has not been bonded to polynucleotide on the surface of the substrate; (3) irradiating the mixture; and (4) measuring the SERS radiation which is generated by the agent captured on the surface of the substrate.

Method for measuring the concentration of polynucleotides

The present invention first provides monoclonal antibodies recognizing membrane phospholipase A.sub.2, namely, monoclonal antibodies PL-49, PL-71, PL-76, and PL-78, hybridomas producing them, methods for producing them, and immunoassays of membrane phospholipase A.sub.2 using them. The immunoassay of PLA.sub.2 M is useful for the diagnosis of articular rheumatism, cancers, and a wide variety of inflammatory states.

Monoclonal antibody recognizing membrane phospholipase A.sub.2 and

The radioactive iodine compound of this invention or an intermediate for preparing the compound is represented by the following formula (I): ##STR1## wherein R is H or .sup.125 I. The radioactive compound can readily introduce a radioactive isotope element such as .sup.125 I into an antibody molecule without damaging the activity of the antibody molecule. Therefore, the compound can preferably be used to label an antibody of immunoradiometric assay, and allow a higher degree of measurement sensitivity.

Radioactive iodine compound for labeling an antibody

The present invention relates to monoclonal antibodies having high affinity to human pancreatic PLA.sub.2, the production thereof, hybridomas producing them and an immunoassay for human pancreatic PLA.sub.2 using them.

Monoclonal antibody specific to human pancreatic phospholipase A.sub.2

A substituted aldosterone of the formula: ##STR1## wherein either one of R.sup.1 and R.sup.2 is hydrogen and the other is --S(CH.sub.2).sub.m COR.sup.3 or --OCO(CH.sub.2).sub.n COR.sup.3, provided that when R.sup.1 is hydrogen, R.sup.2 is --S(CH.sub.2).sub.m COR.sup.3 or --OCO(CH.sub.2).sub.n COR.sup.3 and when R.sup.2 is hydrogen, R.sup.1 is --S(CH.sub.2).sub.m COR.sup.3 ; m being an integer from 1 to 3, n being an integer from 1 to 5 and R.sup.3 being hydroxyl, lower alkoxy or a residue of tyramine, tyrosine lower alkyl ester, histamine, histidine, 7-aminoheptanoyltyrosine lower alkyl ester or .beta.-D-galactosidase as optionally iodinated, or its (18-20)-acetal 20,21-ketonide, which is useful as the reagent in determination of aldosterones by radioimmunoassay or enzyme immunoassay.

4- or 6-substituted aldosterones, their production and use in immunoassay

4- or 6-substituted aldosterones

The disclosed peptide derivative of the formula:

Tyrosylated proinsulin C-peptide derivatives

Growing community of inventors

Osaka, Japan

Masao Kono