Toyo Boseki Kabushiki Kaisha

Toyobo Co., Ltd.

Hiroshima University

Gekkeikan Sake Company Limited

Masao Kitabayashi

Yoshiaki Nishiya

Yoshihisa Kawamura

Tadayuki Imanaka

Bunsei Kawakami

Yuji Tsuji

Hiroshi Kawaminami

Masahiro Takagi

Takahide Kishimoto

Masaaki Morikawa

Hiroaki Inoue

Hideyuki Komatsubara

Taku Arakawa

Toshihiro Kuroita

Yoshikazu Ishida

Shuhei Yamamoto

Hiroshi Aiba

Masanori Oka

Rie Hirao

Yoji Hata

Hideki Kamimura

Hiroki Ishida

Rie Nagai

Naoki Isobe

Keiichi Hisaeda

An object is to provide a novel means for ameliorating mastitis or improving milk production. An ameliorating or therapeutic agent for mastitis that contains a biosurfactant, or a milk production promoting agent that contains a biosurfactant, is provided.

Use of biosurfactant

The present invention provides: a protein having a fructosyl amino acid oxidase activity which protein is useful for measurement of a glycosylated protein (particularly, glycosylated hemoglobin); a modified protein thereof; and use of the protein or the modified protein. The protein of the present invention is, for example, a fructosyl valyl histidine oxidase derived from, the fructosyl valyl histidine oxidase having excellent thermal stability and substrate specificity and also having a small Km value to fructosyl valyl histidine. This allows a glycosylated protein measuring reagent to be stored in a long time and measurement accuracy of the glycosylated protein measuring reagent to be improved.

Protein having fructosyl valyl histidine oxidase activity, modified protein, and use of the protein or the modified protein

The invention relates to a method for improving the heat stability of glucose dehydrogenase (GDH). The invention provides a method for preparing a glucose sensor with soluble flavin-bound GDH by providing a composition comprising soluble flavin-bound GDH at an acidic pH and heat drying the composition onto a glucose sensor.

Method for improving heat stability of composition containing water-soluble coenzyme-bound glucose dehydrogenase (GDH)

Provided is an enzyme that is further advantageous in terms of practical aspects when compared to publicly known enzymes for blood sugar sensors, and that can be used in a blood sugar level measuring reagent A flavin adenine dinucleotide-dependent glucose dehydrogenase that has amino acid sequence including a specific amino acid in an amino acid sequence shown in SEQ ID NO:2 or an amino acid sequence that has 60% homology therewith, and that has an improved temperature dependency.

Modified flavin adenine dinucleotide-dependent glucose dehydrogenase

The invention provides a modified flavin adenine dinucleotide dependent glucose dehydrogenase (FADGDH), as well as a glucose sensor comprising the modified FADGDH and a method for measuring glucose comprising using the glucose sensor to measure glucose of a sample.

Modified flavin adenine dinucleotide dependent glucose dehydrogenase

To overcome disadvantages of a known creatinine amide hydrolase, and provide a creatinine amide hydrolase having improved affinity for creatinine or having a decreased Km value for creatinine, and also provide a reagent composition for use in the determination of creatinine, which is adapted to an automated analysis apparatus and is excellent in accuracy, preciseness and economic efficiency. Disclosed is a modified creatinine amide hydrolase having improved affinity for a substrate compared to an unmodified one. Also disclosed is a creatinine determination reagent comprising the modified creatinine amide hydrolase, a creatinine amidino hydrolase, sarcosine oxidase and a reagent for detection of hydrogen peroxide.

Modified creatinine amide hydrolase having improved affinity for substrate, and reagent composition for determination of creatinine

The present invention provides a method for highly expressing a recombinant FAD-GDH protein derived from filamentous fungi, protein obtained by the method, and a regent for measuring glucose using the protein. According to the invention, the FAD-GDH can be highly expressed by altering DNA sequence coding for a signal peptide of FAD-GDH gene isolated from. FAD-GDH can be stably produced by adjusting pH of 7.1 to 7.3 during culture production.

Method for highly expressing recombinant glucose dehydrogenase derived from filamentous fungi

An object of the present invention is to provide a more practically advantageous enzyme usable as a reagent for measuring blood glucose than the known enzymes used as blood glucose sensors. A modified flavin adenine dinucleotide dependent glucose dehydrogenase (FADGDH) with more improved heat stability than FADGDH derived from wild-type FADGDH, the modified FADGDH being derived from preferably a eukaryote, more preferably a filamentous fungus, and furthermore preferably anfungus, and, for example, those having a primary structure with at least one amino acid substituted, deleted, inserted or added to FADGDH having an amino acid sequence represented by SEQ ID Nos. 2 or 46 in the sequence table.

The present invention effectively produces glucose dehydrogenase derived from, and provides more practical glucose dehydrogenase. The invention makes it possible to efficiently produce glucose dehydrogenase and to obtain glucose dehydrogenase in more practical manner by using a glucose dehydrogenase gene isolated from

[object Object]

The invention relates to a method for improving the heat stability of glucose dehydrogenase (GDH), comprising a step of combining, in a composition comprising soluble coenzyme-bound glucose dehydrogenase (GDH), the enzyme with any one or more selected from the group consisting of sugar alcohols, carboxyl group-containing compounds, alkali metal-containing compounds, alkali earth metal compounds, ammonium salts, sulfate salts and proteins, thereby improving the heat stability of GDH over that achieved without the inclusion of the compounds.

The present invention effectively produces glucose dehydrogenase derived from, and provides more practical glucose dehydrogenase.

An object of the present invention is to provide a thermostable DNA polymerase with enhanced amplification efficiency and/or improved fidelity in polymerase chain reaction (PCR), and provide a process for production thereof. More specifically, the present invention provides thermostable DNA polymerase wherein in the DXEXXXH sequence (D: aspartic acid, E: glutamic acid, H: histidine, X, X, Xand X: any amino acid) consisting of DXE sequence within the EXO I region and a four amino acid length peptide adjacent to said glutamic acid(E) of thermostable DNA polymerase having 3'-5&#x2032; exonuclease activity, histidine(H) has been replaced by another amino acid.

Modified thermostable DNA polymerase

The invention provides methods, kits, and compositions for enhancing synthesis of DNA involving a carboxylate ion-supplying substance that is effective in promoting DNA synthesis in enzymatic DNA synthesis reactions. The invention further provides a thermostable DNA polymerase-related factor derived fromspecies, which has an activity to promote the DNA synthesis activity of DNA polymerase or which binds to DNA polymerase.

Compositions for enhancing DNA synthesis, DNA polymerase-related factors and utilization thereof

The present invention relates to a method for lowering activity with respect to maltose in glucose measurement comprising a step of reacting modified pyrroloquinoline quinone dependent glucose dehydrogenase subjected to amino acid sequence modification, wherein pyrroloquinoline quinone dependent glucose dehydrogenase is reacted in the presence of at least one type of substance selected from the group comprising succinic acid, malonic acid, glutaric acid, malic acid, phthalic acid, 2-ketoglutaric acid, 3,3-dimethylglutaric acid, pimeric acid, suberic acid, adipic acid, maleic acid, potassium chloride, ammonium chloride, diammonium hydrogen citrate, L-lysine, taurine, calcium glycerate, amino-n-butyric acid, sodium glycolate, sodium &#x3b1;-ketoglutarate, fumaric acid, glycine, glutamic acid, serine and citric acid.

Composition for measuring glucose having improved substrate specificity

A nucleic acid amplifying enzyme having a short reaction time and high fidelity is provided. The enzyme of this invention is a thermostable DNA polymerase having a nucleic acid extension rate of at least 30 bases per second and a 3&prime;-5&prime; exonuclease activity. Also provided are a method and kit for amplifying nucleic acid.

Growing community of inventors

Tsuruga, Japan

Masao Kitabayashi